Rabbit Anti-CREB1 (pSer133) Recombinant Antibody (VS3-FY2928) (CAT#: VS3-FY2928)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat CREB1 (pSer133)

• Species Reactivity
• Human, Mouse, Rat

• Applications
• Western Blot, Immunohistochemistry, ELISA

• Conjugate
• Unconjugated, FITC

• MW
• The molecular weight of the target protein is approximately 35 kDa.

• Related Disease
• Histiocytoma, Angiomatoid Fibrous and Melanoma Of Soft Tissue

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/2000
  Immunohistochemistry: 1/50-1/200
  ELISA: 1/1000-1/10000

• Alternative Names
• CAMP Responsive Element Binding Protein 1; CREB-1; Cyclic Adenosine 3,5-Monophosphate Response Element-Binding Protein CREB; Cyclic AMP-Responsive Element-Binding Protein 1; CAMP-Responsive Element-Binding Protein 1; CAMP-Response Element-Binding Protein-1; Active Transcription Factor CREB; Transactivator Protein; CREB

• Gene ID
• 1385

• UniProt ID
• P16220

• Sequence Similarities
• Belongs to the bZIP family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Stimulated by phosphorylation. Phosphorylation of both Ser-119 and Ser-128 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-119 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity).
  CREBL2 positively regulates phosphorylation at Ser-119 thereby stimulating CREB1 transcriptional activity (By similarity).
  Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-119. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-128. Phosphorylation of Ser-128 blocks CREB-mediated transcription even when Ser-119 is phosphorylated. Phosphorylated by CaMK1 (By similarity).
  Phosphorylation of Ser-257 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-119 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. Phosphorylated by TSSK4 on Ser-119 (PubMed:15964553).
  Sumoylated with SUMO1. Sumoylation on Lys-290, but not on Lys-271, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.

• Protein Refseq
• NP_004370.1; NP_604391.1

• Function
• Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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