Rabbit Anti-CREB1 Recombinant Antibody (clone 3C11) (CAT#: ZG-0623J)
This product is a rabbit antibody that recognizes CREB1.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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WB
Figure 1 Rabbit Anti-CREB1 Antibody (ZG-0623J) in WB
Western Blot
Positive WB detected in Hela whole cell lysate,293 whole cell lysate
All lanes Phospho-CREB1 antibody at 1.65μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 KDa
Observed band size: 46 KDa
IHC
Figure 2 Rabbit Anti-CREB1 Antibody (ZG-0623J) in IHC
IHC image of ZG-0623J diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
Figure 3 Rabbit Anti-CREB1 Antibody (ZG-0623J) in IHC
IHC image of ZG-0623J diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 4 Rabbit Anti-CREB1 Antibody (ZG-0623J) in IF
Immunofluorescence staining of Hela cells with ZG-0623J at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-CREB1 (S133)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CREB1
- Species Reactivity
- Human
- Clone
- 3C11
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- CREB; CREB-1; cyclic AMP-responsive element-binding protein 1; active transcription factor CREB; cAMP-response element-binding protein-1; cyclic adenosine 3',5'-monophosphate response element binding protein; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; transactivator protein
- Gene ID
- 1385
- UniProt ID
- P16220
Related Resources
cAMP Signaling Pathway
MAPK Signaling Pathway
PPAR Signaling Pathway
TLR Signaling Pathway
TNF Signaling Pathway
Prostate Cancer
Morphine Addiction
Cocaine Addiction
Amphetamine Addiction
Alcoholism
Cushing Syndrome
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0781 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone DS781AB) | WB, IP | Rabbit IgG |
| MOR-0800 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone DS800AB) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| MOR-4560 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone TH70DS) | WB, IF, ICC, FC, ChIP | Rabbit IgG |
| MOR-4561 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone TH71DS) | WB, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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