Human Anti-EPHA2 Recombinant Antibody (clone G5) (CAT#: HPAB-0164CQ)
This product is a recombinant human antibody that can bind to human EPHA2 with high affinity.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IF
Figure 1 PC3 cells were labeled with either human α-EphA2 mAb (G5; panels A and B) Or R347 isotype control (panel C)
Cell surface attached antibodies were then allowed to Internalize by incubating the cells undergrowth conditions for either zero (non-internalized: panels A and C) or 60 minutes (internalized: panel B). All cells were then fixed (4% Paraformaldehyde), permeablized (0.5% Triton X-100), and stained with AlexaFuor 488-Ab Prior to addition of antifade mounting media and flourescent microscopy examination.
FuncS
Figure 2 Comparison of internalization of several different anti-EphA2 antibodies.
Internalization of the anti EphA2 antibodies B233, B208, EA2, G5, 3F2, 1C1, C2, 3B2 is compared to controls in the following cell lines: PC3, SK Mel-28, HuVec, MCF 10A, and CT26.
Inhib
Figure 3 In vitro growth inhibition of A498 cells, PC-3 cells, and MDA-MB-468 cells by the anti-EphA2 anti body G5 and EA5 linked to MMAF with the vc linker.
Concentrations tested ranged from 0.001 to 100 μg/ml. Results are summarized in three different panels of graphs.
FuncS
Figure 4 In vivo comparison of the anti-EphA2 G5 antibody conjugated to MMAF with the vc linker as com pared to unconjugated G5, control IgG conjugated to MMAF, and control unconjugated IgG in the PC-3 human prostate cancer cell line.
Doses of antibodies were 20 μg and 200 μg.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG1
- Specificity
- Human EPHA2
- Species Reactivity
- Human
- Clone
- G5
- Applications
- ELISA, IF, FC, FuncS, Inter
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunofluorescence, Inhibition and Functional Assay. Please see the Published Data for further information.
Target
- Alternative Names
- EPH Receptor A2; Tyrosine-Protein Kinase Receptor ECK; EC 2.7.10.1; ECK; Epithelial Cell Receptor Protein Tyrosine Kinase; Ephrin Type-A Receptor 2; Soluble EPHA2 Variant 1; Epithelial Cell Kinase;
- Gene ID
- 1969
- UniProt ID
- P29317
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-381MZ | Anti-Human EPHA2 Recombinant Antibody (LUCA40) | FC | |
| TAB-382MZ | Anti-Human EPHA2 Recombinant Antibody (SPL1) | IHC, WB, sELISA | |
| TAB-383MZ | Anti-Human EPHA2 Recombinant Antibody (SG5 ) | IHC, WB, sELISA | |
| TAB-384MZ | Mouse Anti-EPHA2 Recombinant Antibody (TAB-384MZ) | ELISA | Mouse IgG |
| TAB-380MZ-S(P) | Mouse Anti-EPHA2 Recombinant Antibody; scFv Fragment (TAB-380MZ-S(P)) | IHC, WB, sELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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