Mouse Anti-GAPDH Recombinant Antibody (clone 2B8) (CAT#: ZG-0054C)
This product is a mouse antibody that recognizes GAPDH. It can be used in a wide variety of applications, such as: WB, IF/ICC, IHC-P.
Specific Inquiry
IF
Figure 1 Mouse Anti-GAPDH Antibody (clone 2B8) in IF
Immunofluorescence analysis of Hela cell. 1, Cyclin D1 Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). GAPDH Monoclonal Antibody (2B8) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50 min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50 min).
WB
Figure 2 Mouse Anti-GAPDH Antibody (clone 2B8) in WB
WB analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary Antibody was diluted at 1:5000, 4° over night, Secondary antibody (diluted at 1:10000), 37° 1 hour.
IHC
Figure 3 Mouse Anti-GAPDH Antibody (clone 2B8) in IHC
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, GAPDH Monoclonal Antibody (2B8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IHC
Figure 4 Mouse Anti-GAPDH Antibody (clone 2B8) in IHC
mmunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, GAPDH Monoclonal Antibody (2B8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IHC
Figure 5 Mouse Anti-GAPDH Antibody (clone 2B8) in IHC
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, GAPDH Monoclonal Antibody (2B8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only
IF
Figure 6 Mouse Anti-GAPDH Antibody (clone 2B8) in IF
Immunofluorescence analysis of Human-colon tissue. 1, GAPDH Monoclonal Antibody (2B8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
IF
Figure 7 Mouse Anti-GAPDH Antibody (clone 2B8) in IF
Immunofluorescence analysis of Mouse-liver tissue. 1, GAPDH Monoclonal Antibody (2B8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
WB
Figure 8 Mouse Anti-GAPDH Antibody (clone 2B8) in WB
WB analysis of Hela (1), Rat brain (2), Rabbit Muscle (3), Sheep Muscle (4), and Mouse brain (5), diluted at 1:10000.
Specifications
- Immunogen
- Synthetic Peptide of GAPDH
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- GAPDH
- Species Reactivity
- Human, Mouse, Rat, Monkey, Dog, Ch, Hamster, Rabbit, Pig, Sheep, Insect, Yeast
- Clone
- 2B8
- Applications
- WB, IF/ICC, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunofluorescence, Immunohistochemistry-Paraffin. For details, refer to Tested Data.
Target
- Alternative Names
- Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein
- Gene ID
- 2597
- UniProt ID
- P04406
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-386-F(E) | Recombinant Anti-human GAPDH Antibody Fab Fragment | IP, RIA, FuncS | Fab |
| MHH-386-F(E) | Recombinant Human Anti-human GAPDH Antibody Fab Fragment | ELISA, WB, IP, FuncS | Fab |
| FAMAB-0117-YC-F(E) | Human Anti-GAPDH Recombinant Antibody (clone lesion); Fab Fragment | ELISA, WB | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-386-S(P) | Recombinant Human Anti-human GAPDH Antibody scFv Fragment | WB, FC, FuncS | scFv |
| FAMAB-0117-YC-S(P) | Human Anti-GAPDH Recombinant Antibody (clone lesion); scFv Fragment | ELISA, WB | Human scFv |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0217MZ | Chicken Anti-GAPDH Polyclonal IgY | WB | Chicken antibody |
| BRD-0699MZ | Chicken Anti-GAPDH Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1398 | Hi-Affi™ Rabbit Anti-GAPDH Recombinant Antibody (clone DS1398AB) | WB, ICC, IP, FC | Rabbit IgG |
| MOR-0101-FY | Rabbit Anti-GAPDH Recombinant Antibody (clone AFY0072) | IF, IHC-P, WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0630-CN | Mouse Anti-GAPDH Recombinant Antibody (clone 1-G2) | WB, IF | Mouse IgG2b |
| MRO-0631-CN | Rabbit Anti-GAPDH Recombinant Antibody (clone CBACN-235) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-0632-CN | Mouse Anti-GAPDH Recombinant Antibody (clone 5-E10) | WB, IF | Mouse IgM |
| MRO-0633-CN | Rabbit Anti-GAPDH Recombinant Antibody (clone JF81-04), HRP | WB | Rabbit IgG |
| MRO-0202YJ | Mouse Anti-GAPDH Polyclonal Antibody | WB, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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