Mouse Anti-GAPDH Recombinant Antibody (clone 2B8) (CAT#: ZG-0054C)
This product is a mouse antibody that recognizes GAPDH. It can be used in a wide variety of applications, such as: WB, IF/ICC, IHC-P.
Specific Inquiry
IF
Figure 1 Mouse Anti-GAPDH Antibody (clone 2B8) in IF
Immunofluorescence analysis of Hela cell. 1, Cyclin D1 Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). GAPDH Monoclonal Antibody (2B8) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50 min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50 min).
WB
Figure 2 Mouse Anti-GAPDH Antibody (clone 2B8) in WB
WB analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary Antibody was diluted at 1:5000, 4° over night, Secondary antibody (diluted at 1:10000), 37° 1 hour.
IHC
Figure 3 Mouse Anti-GAPDH Antibody (clone 2B8) in IHC
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, GAPDH Monoclonal Antibody (2B8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IHC
Figure 4 Mouse Anti-GAPDH Antibody (clone 2B8) in IHC
mmunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, GAPDH Monoclonal Antibody (2B8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IHC
Figure 5 Mouse Anti-GAPDH Antibody (clone 2B8) in IHC
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, GAPDH Monoclonal Antibody (2B8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only
IF
Figure 6 Mouse Anti-GAPDH Antibody (clone 2B8) in IF
Immunofluorescence analysis of Human-colon tissue. 1, GAPDH Monoclonal Antibody (2B8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
IF
Figure 7 Mouse Anti-GAPDH Antibody (clone 2B8) in IF
Immunofluorescence analysis of Mouse-liver tissue. 1, GAPDH Monoclonal Antibody (2B8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
WB
Figure 8 Mouse Anti-GAPDH Antibody (clone 2B8) in WB
WB analysis of Hela (1), Rat brain (2), Rabbit Muscle (3), Sheep Muscle (4), and Mouse brain (5), diluted at 1:10000.
Specifications
- Immunogen
- Synthetic Peptide of GAPDH
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- GAPDH
- Species Reactivity
- Human, Mouse, Rat, Monkey, Dog, Ch, Hamster, Rabbit, Pig, Sheep, Insect, Yeast
- Clone
- 2B8
- Applications
- WB, IF/ICC, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunofluorescence, Immunohistochemistry-Paraffin. For details, refer to Tested Data.
Target
- Alternative Names
- Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein
- Gene ID
- 2597
- UniProt ID
- P04406
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 2B8"
See other products for "GAPDH"
Select a product category from the dropdown menu below to view related products.
Please select product type
Fab Antibody Products
IgG Antibody Products
ScFv Antibody Products
Chicken IgY Antibody Products
Rabbit Monoclonal Antibody Products
IgM Antibody Products
Antibody Magnetic Beads
Non-Human Primate (NHP) Antibody Products
ScFv-Fc Chimera Products
Anti-Biologic Drug Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-386-F(E) | Recombinant Anti-human GAPDH Antibody Fab Fragment | IP, RIA, FuncS | Fab |
| MHH-386-F(E) | Recombinant Human Anti-human GAPDH Antibody Fab Fragment | ELISA, WB, IP, FuncS | Fab |
| FAMAB-0117-YC-F(E) | Human Anti-GAPDH Recombinant Antibody (clone lesion); Fab Fragment | ELISA, WB | Human Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0054C. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, Cyt, Stim, PP, Agonist
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
-1-1.png)
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: WB, IF, FuncS
-1.png)
Application: ELISA
-1.png)
Application: WB, ELISA, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
