Mouse Anti-GAPDH Recombinant Antibody (VS3-FY2715)
CAT#: VS3-FY2715
This anti-GAPDH product is a mouse recombinant monoclonal antibody that has been carefully developed to target GAPDH. The calculated molecular weight of the protein is 36.1 kDa. It cross-reacts with human, mouse and rat. This monoclonal antibody is potential for conducting research on Microcephaly 21, Primary, Autosomal Recessive and Schistosomiasis. This monoclonal antibody can be used in Western Blot, Immunohistochemistry, Flow cytometry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/752_B3_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach, lower shows strong nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human skin
Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_B_8_1_selected.jpg
Normal Tissue
Figure 5 IHC staining of human kidney
Immunohistochemical staining of human kidney shows cytoplasmic and nuclear positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5197/ihc_selected.jpg
Normal Tissue
Figure 6 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79968/170623_B_3_6_selected.jpg
Subcellular Location
Figure 7 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/1874_F7_61_blue_red_green.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_3.jpg
Normal Tissue
Figure 9 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_5.jpg
Normal Tissue
Figure 11 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_4_6.jpg
Normal Tissue
Figure 12 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_8.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111640-GAPDH
❮
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Glyceraldehyde-3-phosphate dehydrogenase of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 36.1 kDa.
- Related Disease
- Microcephaly 21, Primary, Autosomal Recessive and Schistosomiasis
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/10-1/25
Target
- Alternative Names
- Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein
- Gene ID
- 2597
- UniProt ID
- P04406
- Sequence Similarities
- Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
- Cellular Localization
- Cytoplasm, cytosol
Nucleus
Cytoplasm, perinuclear region
Membrane
Cytoplasm, cytoskeleton
- Post Translation Modifications
- S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity).
S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.
ISGylated.
Sulfhydration at Cys-152 increases catalytic activity.
Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation.
- Protein Refseq
- NP_001243728.1; NP_001276674.1; NP_001276675.1; NP_001344872.1; NP_002037.2
- Function
- Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, 11724794).
Modulates the organization and assembly of the cytoskeleton (By similarity).
Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "GAPDH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-386-F(E) | Recombinant Anti-human GAPDH Antibody Fab Fragment | IP, RIA, FuncS | Fab |
| MHH-386-F(E) | Recombinant Human Anti-human GAPDH Antibody Fab Fragment | ELISA, WB, IP, FuncS | Fab |
| FAMAB-0117-YC-F(E) | Human Anti-GAPDH Recombinant Antibody (clone lesion); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-386 | Recombinant Human Anti-human GAPDH Antibody | ELISA, WB, FC, FuncS | IgG |
| MRO-1883-CN | Rabbit Anti-GAPDH Polyclonal Antibody (MRO-1883-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1884-CN | Rabbit Anti-GAPDH Polyclonal Antibody (MRO-1884-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1885-CN | Rabbit Anti-GAPDH Polyclonal Antibody (MRO-1885-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0053C | Mouse Anti-GAPDH Recombinant Antibody (clone 2B8-1) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-386-S(P) | Recombinant Human Anti-human GAPDH Antibody scFv Fragment | WB, FC, FuncS | scFv |
| FAMAB-0117-YC-S(P) | Human Anti-GAPDH Recombinant Antibody (clone lesion); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0217MZ | Chicken Anti-GAPDH Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1398 | Hi-Affi™ Rabbit Anti-GAPDH Recombinant Antibody (clone DS1398AB) | WB, ICC, IP, FC | Rabbit IgG |
| MOR-0101-FY | Rabbit Anti-GAPDH Recombinant Antibody (clone AFY0072) | IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0632-CN | Mouse Anti-GAPDH Recombinant Antibody (clone 5-E10) | WB, IF | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY108 | AbPlus™ Anti-GAPDH Magnetic Beads (VS-0424-XY108) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY212 | Mouse Anti-NHP GAPDH Recombinant Antibody (clone 1D4) | WB, IF | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY39 | Mouse Anti-GAPDH (clone W25lesion) scFv-Fc Chimera | ELISA, WB | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1224-YC534 | DrugMonitor™ Anti-GAPDH Antibody (VS-1224-YC534) | ELISA, PK/PD, ADA control | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY896 | Anti-GAPDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY2729 | Anti-Mouse GAPDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY2728 | Anti-Human GAPDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY2730 | Anti-Goat GAPDH Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC435 | CytoStream™ Rabbit Anti-GAPDH Recombinant Antibody (VS13-YC435) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
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