Human Anti-HCV Recombinant Antibody (clone CM3.B6) (CAT#: FAMAB-0025WJ)
This product is a human antibody hepatitis C virus core. This antibody can be used in applications, such as ELISA, IF, and IHC.
Specific Inquiry
ELISA
Figure 1 Specificity of hMAbCM3.B6(M) determined by ELISA.
A commercial broad-spectrum assay incorporating recombinant proteins derived fromcore, NS3, and NS4 regions of HCV was also utilized for comparison.
Mondelli, M. U., Cerino, A., Boender, P., Oudshoorn, P., Middeldorp, J., Fipaldini, C., ... & Habets, W. (1994). Significance of the immune response to a major, conformational B-cell epitope on the hepatitis C virus NS3 region defined by a human monoclonal antibody. Journal of virology, 68(8), 4829-4836.
WB
Figure 2 Western blot analysis of hMAb CM3.B6.
Lanes indicated with NS3 were loaded with truncated NS3 fusion protein (aa 1187 to 1496), whereas lanes indicated with pT7 were loaded with totally sates of bacteria transfected with plasmid pT7-7(28).
Mondelli, M. U., Cerino, A., Boender, P., Oudshoorn, P., Middeldorp, J., Fipaldini, C., ... & Habets, W. (1994). Significance of the immune response to a major, conformational B-cell epitope on the hepatitis C virus NS3 region defined by a human monoclonal antibody. Journal of virology, 68(8), 4829-4836.
IF
Figure 3 Indirect immunofluorescence staining with hMAbCM3.B6 of HeLa cells transfected with pCD(38-9.4).
Notice the exclusive cytoplasmic distribution of the fluorescence.
Mondelli, M. U., Cerino, A., Boender, P., Oudshoorn, P., Middeldorp, J., Fipaldini, C., ... & Habets, W. (1994). Significance of the immune response to a major, conformational B-cell epitope on the hepatitis C virus NS3 region defined by a human monoclonal antibody. Journal of virology, 68(8), 4829-4836.
Inhib
Figure 4 Competitive inhibition of hMAbCM3.B6 binding to recombinant NS3Ag by 44 sera from patients exposed to HCV.
An ELISA was developed for the detection of human antibodies specific for NS3, using purified recombinant, B-Gal-c33c as an antigen.
Mondelli, M. U., Cerino, A., Boender, P., Oudshoorn, P., Middeldorp, J., Fipaldini, C., ... & Habets, W. (1994). Significance of the immune response to a major, conformational B-cell epitope on the hepatitis C virus NS3 region defined by a human monoclonal antibody. Journal of virology, 68(8), 4829-4836.
Inhib
Figure 5 Binding of anti-c33c positive human sera to recombinant NS3 Ag is inhibited by a singlesynthetic peptide, 377, encompassing the epitope defined by hMAbCM3.B6.
Binding of sera to P-Gal-c33c on the solid phase was measured in theabsence and in the presence of 100 ug of synthetic peptide.
Mondelli, M. U., Cerino, A., Boender, P., Oudshoorn, P., Middeldorp, J., Fipaldini, C., ... & Habets, W. (1994). Significance of the immune response to a major, conformational B-cell epitope on the hepatitis C virus NS3 region defined by a human monoclonal antibody. Journal of virology, 68(8), 4829-4836.
Specifications
- Immunogen
- HCV protein
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- HCV core
- Species Reactivity
- HCV
- Clone
- CM3.B6
- Applications
- ELISA, IF, IHC, WB, Inhib
- Related Disease
- HCV infection
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HCV antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry, Western Blot, Inhibition.
Target
- Alternative Names
- Hepatitis C virus; HCV
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-079 | Recombinant Anti-HCV VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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