Rabbit Anti-HMGB1 Recombinant Antibody (clone 1A1) (CAT#: ZG-0740J)
This product is a rabbit antibody that recognizes HMGB1.
Specific Inquiry
WB
Figure 1 Rabbit Anti-HMGB1 Antibody (ZG-0740J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate
All lanes: HMGB1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
IHC
Figure 2 Rabbit Anti-HMGB1 Antibody (ZG-0740J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human HMGB1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HMGB1
- Species Reactivity
- Human
- Clone
- 1A1
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Tumor
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- HMG1; HMG3; HMG-1; SBP-1; high mobility group protein B1; Amphoterin; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1
- Gene ID
- 3146
- UniProt ID
- P09429
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "HMGB1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-956 | Human Anti-HMGB1 Recombinant Antibody (clone Giby-1-4) | ELISA, WB | Human IgG |
| DrMAB-957 | Human Anti-HMGB1 Recombinant Antibody (clone Giby-1-4), Biotin | ELISA, WB | Human IgG |
| MRO-1938-CN | Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1938-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1939-CN | Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1939-CN) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-0101-WJ | Human Anti-HMGB1 Recombinant Antibody (HPAB-0101-WJ) | ELISA, WB | Humanized IgG1, λ |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0315CL-S(P) | Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-0315CL-S(P)) | ELISA, Inhib, FuncS, WB | Mouse scFv |
| TAB-0316CL-S(P) | Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-0316CL-S(P)) | ELISA, Inhib, FuncS, WB | Mouse scFv |
| TAB-0317CL-S(P) | Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-0317CL-S(P)) | ELISA, Inhib, FuncS, WB | Mouse scFv |
| TAB-0318CL-S(P) | Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-0318CL-S(P)) | ELISA, Inhib, FuncS, WB | Mouse scFv |
| TAB-100CT-S(P) | Anti-Human HMG1 Recombinant Antibody scFv Fragment (DPH1 .1 mAb) | IF, FC |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-099CT | Human Anti-HMGB1 Recombinant Antibody (TAB-099CT) | IF, ELISA, IHC | Human IgG1, λ |
| TAB-099CT-S(P) | Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-099CT-S(P)) | ELISA, FC, Block | Human scFv |
| TAB-099CT-F(E) | Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (TAB-099CT-F(E)) | ELISA, FC, Block | Humanized Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-101CT | Anti-Human HMG1 Recombinant Antibody | ELISA, BL | Human antibody |
| TAB-101CT-S(P) | Anti-Human HMG1 Recombinant Antibody scFv Fragment | ELISA, BL | Human antibody |
| TAB-101CT-F(E) | Anti-Human HMG1 Recombinant Antibody Fab Fragment | ELISA, BL | Human antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1633 | Rabbit Anti-HMGB1 Recombinant Antibody (clone DS1633AB) | WB, IHC-P, FC | Rabbit IgG |
| MOR-0049-FY | Rabbit Anti-HMGB1 Recombinant Antibody (clone AFY0020) | ICC, IHC-P, WB | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2082 | PE-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer | FCM | |
| MHC-LC2083 | APC-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer | FCM |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0215-FY-S(P) | Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); scFv Fragment | ELISA | Mouse scFv |
| HPAB-N0137-YC-S(P) | Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-N0137-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-0101-WJ-S(P) | Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-0101-WJ-S(P)) | ELISA, WB | Humanized scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0215-FY-F(E) | Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); Fab Fragment | ELISA | Mouse Fab |
| HPAB-N0137-YC-F(E) | Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-N0137-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-0101-WJ-F(E) | Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-0101-WJ-F(E)) | ELISA, WB | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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