Human Anti-IGF2 Recombinant Antibody (clone m610.27) (CAT#: HPAB-0613-WJ)

Figure 1 Competition of m610 with m708.5 in binding to IGF2.

IgG1 m610.27 was coated on the ELISA plate and IGF2 was captured by coated IgG1 m610.27. Bound scFv m708.5 or VH m630.3 was detected by HRP-conjugated anti-Flag tag antibody.

Feng, Y., Zhao, Q., Chen, W., Wang, Y., Crowder, K., & Dimitrov, D. S. (2014). A new bispecific antibody targeting non-overlapping epitopes on IGF2: design, in vitro characterization and pharmacokinetics in macaques. Experimental and molecular pathology, 97(3), 359-367.

Figure 2 m67 inhibited bindings of IGF2 to MCF7 cells.

MCF-7 cells were incubated with 1 nM biotinylated IGF2 in the absence or presence of antibodies. Bound biotinylated 2 was detected by streptavidin-PE. Cells incubated with streptavidin-PE conjugate only as the background control are in black. Cells incubated with biotin-IGF2 are in red. Those for biotin-IGF2 with antibodies are in blue.

Feng, Y., Zhao, Q., Chen, W., Wang, Y., Crowder, K., & Dimitrov, D. S. (2014). A new bispecific antibody targeting non-overlapping epitopes on IGF2: design, in vitro characterization and pharmacokinetics in macaques. Experimental and molecular pathology, 97(3), 359-367.

Figure 3 Inhibition of IGF1R and IR phosphorylation.

MCF-7 cells were starved in serum free medium for 5 h first, followed by addition of treatment medium with 1 nM IGF1 or 5 nM IGF2 with indicated concentrations of antibodies. Thirty minutes later cells were chilled and lysed. IGF1R was immunoprecipitated and the phosphorylated IGF1R was detected with a phosphor-tyrosine specific antibody. The total amount of IGF1R were detected by the m610.27 antibody used for the immunoprecipitation.

Feng, Y., Zhao, Q., Chen, W., Wang, Y., Crowder, K., & Dimitrov, D. S. (2014). A new bispecific antibody targeting non-overlapping epitopes on IGF2: design, in vitro characterization and pharmacokinetics in macaques. Experimental and molecular pathology, 97(3), 359-367.