Mouse Anti-IL18 Recombinant Antibody (ZG-0496J)
CAT#: ZG-0496J
This product is a mouse antibody that recognizes IL18.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-IL18 Recombinant Antibody (ZG-0496J) in WB
Western blot detection of IL18 binding protein in CHO-K1 cells transfected by IL18BP-PDGFR fusion protein and K562 cell lysates using IL18 binding protein mouse mAb (1:400-1:1000 diluted). Predicted band size:37KDa.Observed band size:37/40KDa.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/16_G12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/14_G12_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/13_G12_1_red_green.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (microvilli)
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_9_5.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HaCaT, HBEC3-KT, hTCEpi, PC-3, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150782-IL18
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Specifications
- Immunogen
- Purified recombinant human IL18 binding protein protein fragments expressed in E.coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human IL18
- Species Reactivity
- Human
- Applications
- WB
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot.
Target
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Related Diseases
Inflammatory Bowel Diseases
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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