Human Anti-IL1RAP Recombinant Antibody (HPAB-M0109-YC)
CAT#: HPAB-M0109-YC
This product is a recombinant Human antibody that recognizes interleukin-1 receptor accessory protein. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/372_B3_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subsets of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Peripheral nerve/ganglion
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196083-IL1RAP
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human IL1RAP
- Species Reactivity
- Human
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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High Quality Humanized Antibody
This recombinant Human Anti-IL1RAP antibody worked perfectly in our ELISA setup. As a humanized IgG, it offered the specific binding characteristics we needed to study the interleukin-1 receptor accessory protein. The purity, confirmed to be greater than 95% by SDS-PAGE, resulted in very clean data for our research reports.
Consistent Recombinant Production
We value the consistency provided by this recombinant antibody production method. Using chemically defined culture media for expression eliminates animal-derived contaminants. We have used this product for multiple rounds of ELISA to detect IL1RAP, and the results have been highly reproducible between different lots.
Q&As
-
What is the format and target of this recombinant antibody?
A: This is a recombinant Humanized antibody that specifically recognizes the Human interleukin-1 receptor accessory protein (IL1RAP). It is expressed in mammalian cells and purified via affinity chromatography. It is an IgG isotype suitable for various research needs.
-
Which immunoassay techniques can this antibody be used for?
A: This Anti-IL1RAP Recombinant Antibody has been tested and validated for use in Enzyme-linked Immunosorbent Assay (ELISA). It is designed to detect the accessory protein that forms a complex with the interleukin 1 receptor at the cell membrane.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| HPAB-M0109-YC-S(P) | Human Anti-IL1RAP Recombinant Antibody; scFv Fragment (HPAB-M0109-YC-S(P)) | ELISA | Human scFv |
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| HPAB-M0110-YC-S(P) | Human Anti-IL1RAP Recombinant Antibody; scFv Fragment (HPAB-M0110-YC-S(P)) | ELISA | Human scFv |
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| HPAB-M0111-YC-S(P) | Human Anti-IL1RAP Recombinant Antibody; scFv Fragment (HPAB-M0111-YC-S(P)) | ELISA | Human scFv |
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| HPAB-M0109-YC-F(E) | Human Anti-IL1RAP Recombinant Antibody; Fab Fragment (HPAB-M0109-YC-F(E)) | ELISA | Humanized Fab |
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| HPAB-M0110-YC-F(E) | Human Anti-IL1RAP Recombinant Antibody; Fab Fragment (HPAB-M0110-YC-F(E)) | ELISA | Humanized Fab |
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| HPAB-M0111-YC-F(E) | Human Anti-IL1RAP Recombinant Antibody; Fab Fragment (HPAB-M0111-YC-F(E)) | ELISA | Humanized Fab |
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