Rabbit Anti-INS Recombinant Antibody (clone 1F6)
CAT#: ZG-0769J
This product is a rabbit antibody that recognizes INS.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-INS Antibody (ZG-0769J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 2 Rabbit Anti-INS Antibody (ZG-0769J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Normal Tissue
Figure 1 Pancreas
Pancreatic endocrine cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4932/115849_A_1_3.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (EFO-21, U-2 OS, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000254647-INS
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Specifications
- Immunogen
- A synthesized peptide derived from human Insulin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human INS
- Species Reactivity
- Human
- Clone
- 1F6
- Applications
- ELISA, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
IHC:1:50-1:200
Target
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Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "INS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1554-VHH | Recombinant Anti-human INS VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1841 | Rabbit Anti-INS Recombinant Antibody (clone DS1841AB) | IHC-P, WB | Rabbit IgG |
| MOR-4627 | Rabbit Anti-INS Recombinant Antibody (clone TH140DS) | IF, ICC, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF029 | DRB1*04:01/Human Proinsulin (GAGSLQPLALEGSLQKRG) MHC Monomer | MHC Multimer | |
| MHC-YF032 | DRB1*04:01/Human Proinsulin (GIVEQCCTSICSLYQ) MHC Monomer | MHC Multimer | |
| MHC-YF236 | A*02:01/Human Ins B. 10 (HLVEALYLV) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0352JF | Mouse Anti-INS Recombinant Antibody (clone CG7C7) | ELISA, FuncS | Mouse IgG1 |
| FAMAB-0353JF | Mouse Anti-INS Recombinant Antibody (clone AE9D6) | ELISA, FuncS | Mouse IgG1 |
| FAMAB-0354JF | Mouse Anti-INS Recombinant Antibody (clone CB5) | ELISA, FuncS | Mouse IgG1 |
| FAMAB-0355JF | Mouse Anti-INS Recombinant Antibody (clone CA4) | ELISA, FuncS | Mouse IgG2b |
| FAMAB-0356JF | Mouse Anti-INS Recombinant Antibody (clone BE3F9) | ELISA, FuncS | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0352JF-F(E) | Mouse Anti-INS Recombinant Antibody (clone CG7C7); Fab Fragment | ELISA, FuncS | Mouse Fab |
| FAMAB-0353JF-F(E) | Mouse Anti-INS Recombinant Antibody (clone AE9D6); Fab Fragment | ELISA, FuncS | Mouse Fab |
| FAMAB-0354JF-F(E) | Mouse Anti-INS Recombinant Antibody (clone CB5); Fab Fragment | ELISA, FuncS | Mouse Fab |
| FAMAB-0355JF-F(E) | Mouse Anti-INS Recombinant Antibody (clone CA4); Fab Fragment | ELISA, FuncS | Mouse Fab |
| FAMAB-0356JF-F(E) | Mouse Anti-INS Recombinant Antibody (clone BE3F9); Fab Fragment | ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0352JF-S(P) | Mouse Anti-INS Recombinant Antibody (clone CG7C7); scFv Fragment | ELISA, FuncS | Mouse scFv |
| FAMAB-0353JF-S(P) | Mouse Anti-INS Recombinant Antibody (clone AE9D6); scFv Fragment | ELISA, FuncS | Mouse scFv |
| FAMAB-0354JF-S(P) | Mouse Anti-INS Recombinant Antibody (clone CB5); scFv Fragment | ELISA, FuncS | Mouse scFv |
| FAMAB-0355JF-S(P) | Mouse Anti-INS Recombinant Antibody (clone CA4); scFv Fragment | ELISA, FuncS | Mouse scFv |
| FAMAB-0356JF-S(P) | Mouse Anti-INS Recombinant Antibody (clone BE3F9); scFv Fragment | ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3566 | Anti-INS Immunohistochemistry Kit | IHC | |
| VS-0525-XY3567 | Anti-Mouse INS Immunohistochemistry Kit | IHC | |
| VS-0525-XY3568 | Anti-Rat INS Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC112 | Recombinant Anti-INS (α chain x β chain) Biparatopic Antibody, Tandem scFv (Clone AE9D6 x Clone CB5) | ELISA | Tandem scFv |
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