Rabbit Anti-ITGAV Recombinant Antibody (clone 5G3) (CAT#: ZG-0041U)
The purified Rabbit Anti-ITGAV Recombinant Antibody (clone 5G3) displayed high specificity against the ITGAV protein of human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-ITGAV Antibody (ZG-0041U) in WB
Positive WB detected in: U-251 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate. All lanes: ITGAV antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 117, 113, 112 kDa
Observed band size: 130 kDa
IHC
Figure 2 Rabbit Anti-ITGAV Antibody (ZG-0041U) in IHC
IHC image of ZG-0041U diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Integrin alpha V
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ITGAV
- Species Reactivity
- Human
- Clone
- 5G3
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- West Nile Virus and Herpes Simplex
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Integrin alpha-V; Vitronectin receptor; Vitronectin receptor subunit alpha; CD antigen CD51; Cleaved into: Integrin alpha-V heavy chain; Integrin alpha-V light chain; ITGAV; MSK8; VNRA; VTNR
- Gene ID
- 3685
- UniProt ID
- P06756
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-076 | Anti-Human CD51 Recombinant Antibody (TAB-076) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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