Mouse Anti-ITGB1 Recombinant Antibody (clone 1G9-B0-F5)
CAT#: VS3-QX610
This product is a mouse antibody that recognizes ITGB1. The antibody can be used in applications: WB, IHC, ICC, FC, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3434/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69003/165766_B_5_7_selected.jpg
Normal Tissue
Figure 3 Immunofluorescent staining of human cell line BJ
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3434/ihc_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59297/139504_A_7_5.jpg
Normal Tissue
Figure 5 Smooth muscle
Smooth muscle cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59297/139504_B_6_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150093-ITGB1
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Specifications
- Immunogen
- Purified recombinant fragment of human ITGB1 expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Integrin β1
- Species Reactivity
- Human, Monkey
- Clone
- 1G9-B0-F5
- Applications
- WB, IHC, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1:500-1:2000; IHC: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000
Target
REVIEWS AND Q&AS
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-772LC | Anti-human ITGB1 Recombinant Antibody (1B4) (TAB-772LC) | FuncS | |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1586CQ | Recombinant Mouse Anti-ITGB1 Antibody (P4C10) | WB, Neut, ELISA, FC, ICC, IF, IHC-Fr, IP | IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-047CN | Human Anti-ITGB1 Recombinant Antibody | ELISA, WB, IHC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-047CN-F(E) | Human Anti-ITGB1 Recombinant Antibody; Fab Fragment | ELISA, WB, IHC | Human Fab |
| HPAB-0253CQ-F(E) | Mouse Anti-ITGB1 Recombinant Antibody (clone HUTS-21); Fab Fragment | FC | Mouse Fab |
| HPAB-0254CQ-F(E) | Rat Anti-ITGB1 Recombinant Antibody (clone MAb AIIB2); Fab Fragment | ELISA, FC, WB, ICC, Block, IP, IHC | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-047CN-S(P) | Human Anti-ITGB1 Recombinant Antibody; scFv Fragment | ELISA, WB, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY161 | AbPlus™ Anti-ITGB1 Magnetic Beads (P4C10) | IP, Protein Purification | |
| VS-0724-YC1500 | AbPlus™ Anti-Itgb1 Magnetic Beads (VS-0724-YC1500) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY289 | Mouse Anti-NHP ITGB1 Recombinant Antibody (clone 3B6) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY152 | CytoStream™ Mouse Anti-ITGB1 Recombinant Antibody (VS-0225-XY152) | FC | Mouse IgG1, kappa |
| VS13-YC603 | CytoStream™ Rabbit Anti-ITGB1 Recombinant Antibody (VS13-YC603) | WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1156 | Anti-ITGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3653 | Anti-Mouse ITGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3655 | Anti-Human ITGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3654 | Anti-Porcine ITGB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC597 | Recombinant Anti-ITGB1 Vesicular Antibody, EV Displayed (VS-0425-YC597) | Block, ELISA, FC, Cell-uptake |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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