Rabbit Anti-ITGB1 Recombinant Antibody (clone 32A10)
CAT#: ZG-0776J
This product is a rabbit antibody that recognizes ITGB1.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-ITGB1 Antibody (ZG-0776J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, U251 whole cell lysate
All lanes: Integrin beta-1/CD29 antibody at 1.9 μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 89, 88, 92 KDa
Observed band size: 130 KDa
IHC
Figure 2 Rabbit Anti-ITGB1 Antibody (ZG-0776J) in IHC
IHC image of this product diluted at 1:185 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-ITGB1 Antibody (ZG-0776J) in IF
Immunofluorescence staining of Hela cells with this product at 1:61, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
FC
Figure 4 Rabbit Anti-ITGB1 Antibody (ZG-0776J) in FC
Overlay histogram showing MCF-7 cells stained with this product (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3434/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69003/165766_B_5_7_selected.jpg
Normal Tissue
Figure 3 Immunofluorescent staining of human cell line BJ
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3434/ihc_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59297/139504_A_7_5.jpg
Normal Tissue
Figure 5 Smooth muscle
Smooth muscle cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59297/139504_B_6_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150093-ITGB1
❮
❯
Specifications
- Immunogen
- A synthesized peptide derived from human ITGB1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ITGB1
- Species Reactivity
- Human
- Clone
- 32A10
- Applications
- ELISA, WB, IHC, IF, FC
- Conjugate
- Uconjugated
- Related Disease
- Embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA; integrin beta-1; glycoprotein IIa; integrin VLA-4 beta subunit; integrin beta 1; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); very late activation protein, beta polypeptide
- Gene ID
- 3688
- UniProt ID
- P05556
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0776J. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ITGB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1187z | Mouse Anti-ITGB1 Recombinant Antibody (clone 20C3) | ELISA, FC, IHC, WB | Mouse IgG1 |
| HPAB-0253CQ | Mouse Anti-ITGB1 Recombinant Antibody (clone HUTS-21) | FC | Mouse IgG2a, κ |
| HPAB-0254CQ | Rat Anti-ITGB1 Recombinant Antibody (clone MAb AIIB2) | ELISA, FC, WB, ICC, Block, IP, IF, IHC | Rat IgG1, κ |
| VS3-QX609 | Mouse Anti-ITGB1 Recombinant Antibody (clone 7B5-C4-A5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX610 | Mouse Anti-ITGB1 Recombinant Antibody (clone 1G9-B0-F5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-772LC | Anti-human ITGB1 Recombinant Antibody (1B4) (TAB-772LC) | FuncS | |
| TAB-772LC-S(P) | Anti-human ITGB1 scFv Fragment (1B4) | ELISA | |
| TAB-772LC-F(E) | Anti-human ITGB1 Fab Fragment (1B4) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0277MZ | Chicken Anti-Integrin β1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1585CQ | Recombinant Mouse Anti-ITGB1 Antibody (CBL252) | FC, ELISA, WB, IP, FuncS, Inhib | IgG1 |
| NEUT-1587CQ | Recombinant Mouse Anti-ITGB1 Antibody (P5D2) | BL, FC, IP, CyTOF, ICC | IgG1 |
| NEUT-1588CQ | Recombinant Armenian Hamster Anti-Itgb1 Antibody (HMβ1-1) | FC, IP, BL, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1586CQ | Recombinant Mouse Anti-ITGB1 Antibody (P4C10) | WB, Neut, ELISA, FC, ICC, IF, IHC-Fr, IP | IgG1 |
| NEUT-1589CQ | Recombinant Armenian hamster Anti-Itgb1 Antibody (HMb1-1) | FC, FuncS, Neut | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1882 | Hi-Affi™ Recombinant Rabbit Anti-ITGB1 Monoclonal Antibody (DS1882AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-047CN | Human Anti-ITGB1 Recombinant Antibody | ELISA, WB, IHC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-047CN-F(E) | Human Anti-ITGB1 Recombinant Antibody; Fab Fragment | ELISA, WB, IHC | Human Fab |
| HPAB-0253CQ-F(E) | Mouse Anti-ITGB1 Recombinant Antibody (clone HUTS-21); Fab Fragment | FC | Mouse Fab |
| HPAB-0254CQ-F(E) | Rat Anti-ITGB1 Recombinant Antibody (clone MAb AIIB2); Fab Fragment | ELISA, FC, WB, ICC, Block, IP, IHC | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-047CN-S(P) | Human Anti-ITGB1 Recombinant Antibody; scFv Fragment | ELISA, WB, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY161 | AbPlus™ Anti-ITGB1 Magnetic Beads (P4C10) | IP, Protein Purification | |
| VS-0724-YC1500 | AbPlus™ Anti-Itgb1 Magnetic Beads (VS-0724-YC1500) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC53 | Rabbit Anti-ITGB1 Antibody (VS-0924-YC53) - Cancer Stem Cell Marker | IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY289 | Mouse Anti-NHP ITGB1 Recombinant Antibody (clone 3B6) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY152 | CytoStream™ Mouse Anti-ITGB1 Recombinant Antibody (VS-0225-XY152) | FC | Mouse IgG1, kappa |
| VS13-YC603 | CytoStream™ Rabbit Anti-ITGB1 Recombinant Antibody (VS13-YC603) | WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1156 | Anti-ITGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3653 | Anti-Mouse ITGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3655 | Anti-Human ITGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3654 | Anti-Porcine ITGB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC597 | Recombinant Anti-ITGB1 Vesicular Antibody, EV Displayed (VS-0425-YC597) | Block, ELISA, FC, Cell-uptake |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
-2.png)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, IF, IP, FC, FuncS
Application: ELISA, IHC, WB
Application: ELISA, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
