This product is a rabbit antibody that recognizes ITGB1.
Figure 1 Rabbit Anti-ITGB1 Antibody (ZG-0776J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, U251 whole cell lysate
All lanes: Integrin beta-1/CD29 antibody at 1.9 μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 89, 88, 92 KDa
Observed band size: 130 KDa
Figure 2 Rabbit Anti-ITGB1 Antibody (ZG-0776J) in IHC
IHC image of this product diluted at 1:185 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Figure 3 Rabbit Anti-ITGB1 Antibody (ZG-0776J) in IF
Immunofluorescence staining of Hela cells with this product at 1:61, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Figure 4 Rabbit Anti-ITGB1 Antibody (ZG-0776J) in FC
Overlay histogram showing MCF-7 cells stained with this product (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1187z | Mouse Anti-ITGB1 Recombinant Antibody (clone 20C3) | ELISA, FC, IHC, WB | Mouse IgG1 |
HPAB-0253CQ | Mouse Anti-ITGB1 Recombinant Antibody (clone HUTS-21) | FC | Mouse IgG2a, κ |
HPAB-0254CQ | Rat Anti-ITGB1 Recombinant Antibody (clone MAb AIIB2) | ELISA, FC, WB, ICC, Block, IP, IF, IHC | Rat IgG1, κ |
VS3-QX609 | Mouse Anti-ITGB1 Recombinant Antibody (clone 7B5-C4-A5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
VS3-FY775 | Recombinant Rabbit Anti-ITGB1 Antibody (clone R06-1H5) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-772LC | Anti-human ITGB1 Recombinant Antibody (1B4) (TAB-772LC) | FuncS | |
TAB-772LC-S(P) | Anti-human ITGB1 scFv Fragment (1B4) | ELISA | |
TAB-772LC-F(E) | Anti-human ITGB1 Fab Fragment (1B4) | ELISA |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0277MZ | Chicken Anti-Integrin β1 Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1585CQ | Recombinant Mouse Anti-ITGB1 Antibody (CBL252) | FC, ELISA, WB, IP, FuncS, Inhib | IgG1 |
NEUT-1587CQ | Recombinant Mouse Anti-ITGB1 Antibody (P5D2) | BL, FC, IP, CyTOF, ICC | IgG1 |
NEUT-1588CQ | Recombinant Armenian Hamster Anti-Itgb1 Antibody (HMβ1-1) | FC, IP, BL, IHC | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1586CQ | Recombinant Mouse Anti-ITGB1 Antibody (P4C10) | WB, Neut, ELISA, FC, ICC/IF, IHC-Fr, IP | IgG1 |
NEUT-1589CQ | Recombinant Armenian hamster Anti-Itgb1 Antibody (HMb1-1) | FC, FuncS, Neut | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1882 | Hi-Affi™ Recombinant Rabbit Anti-ITGB1 Monoclonal Antibody (DS1882AB) | WB | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-047CN | Human Anti-ITGB1 Recombinant Antibody | ELISA, WB, IHC | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-047CN-F(E) | Human Anti-ITGB1 Recombinant Antibody; Fab Fragment | ELISA, WB, IHC | Human Fab |
HPAB-0253CQ-F(E) | Mouse Anti-ITGB1 Recombinant Antibody (clone HUTS-21); Fab Fragment | FC | Mouse Fab |
HPAB-0254CQ-F(E) | Rat Anti-ITGB1 Recombinant Antibody (clone MAb AIIB2); Fab Fragment | ELISA, FC, WB, ICC, Block, IP, IHC | Rat Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-047CN-S(P) | Human Anti-ITGB1 Recombinant Antibody; scFv Fragment | ELISA, WB, IHC | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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