Rabbit Anti-JUN Recombinant Antibody (clone 10D4) (CAT#: ZG-0783J)
This product is a rabbit antibody that recognizes JUN.
Specific Inquiry
WB
Figure 1 Rabbit Anti-JUN Antibody (ZG-0783J) in WB
Western Blot
Positive WB detected in: 293 whole cell lysate
All lanes: JUN antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 43 kDa
IHC
Figure 2 Rabbit Anti-JUN Antibody (ZG-0783J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human c-Jun
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human JUN
- Species Reactivity
- Human
- Clone
- 10D4
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- AP1; p39; AP-1; cJUN; c-Jun; transcription factor AP-1; Jun activation domain binding protein; activator protein 1; enhancer-binding protein AP1; jun oncogene; proto-oncogene c-Jun; proto-oncogene cJun; v-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog
- Gene ID
- 3725
- UniProt ID
- P05412
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "JUN"
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0043MZ | Chicken Anti-AP-1 Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1902 | Hi-Affi™ Recombinant Rabbit Anti-JUN Monoclonal Antibody (DS1902AB) | IP, WB | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0365-CN | Recombinant Rabbit Anti-JUN Monoclonal Antibody (CBACN-148) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0425F | Mouse Anti-JUN Recombinant Antibody (ZG-0425F) | WB | Mouse IgG |
| VS3-QX619 | Mouse Anti-JUN Recombinant Antibody (clone 10G2-2G3-1F4) | IHC | Mouse IgG1 |
| VS3-QX620 | Mouse Anti-JUN Recombinant Antibody (clone 6A3-7A8-8H3) | IHC | Mouse IgG1 |
| VS3-QX621 | Mouse Anti-JUN Recombinant Antibody (clone 6H6-4D9-2G5) | IHC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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