Mouse Anti-L1CAM Recombinant Antibody (clone L1-14.10) (CAT#: FAMAB-1631CQ)

Figure 1 Comparison of antibody reactivity by fluorescent staining andFACS analysis.

The indicated cell lines were stained with mAb L1-11A or the new mAb L1-14.10 followed by PE-conjugated anti-mouse IgG. Note that L1-14.10 only weakly reacts with L1 by FACS analysis

Huszar, M., Moldenhauer, G., Gschwend, V., Ben-Arie, A., Altevogt, P., & Fogel, M. (2006). Expression profile analysis in multiple human tumors identifies L1 (CD171) as a molecular marker for differential diagnosis and targeted therapy. Human pathology, 37(8), 1000-1008.

Figure 2 Comparison of antibody reactivity by Western blot analysis.

Cell lysates were prepared and separated by SDS-PAGE under reducing conditions. Blots were developed with peroxidase-conjugated goat anti-mouse IgG and enhanced chemiluminescence detection. The L1-220 band (full-length L1) is indicated.

Huszar, M., Moldenhauer, G., Gschwend, V., Ben-Arie, A., Altevogt, P., & Fogel, M. (2006). Expression profile analysis in multiple human tumors identifies L1 (CD171) as a molecular marker for differential diagnosis and targeted therapy. Human pathology, 37(8), 1000-1008.

Figure 3 Immunohistochemistry staining of ovarian carcinoma tissue section.

Immunohistochemistry staining of ovarian carcinoma tissue section using L1-specific mAb L1-11A and L1-14.10. Note the brighter staining of the newly developed mAb L1-14.10.

Huszar, M., Moldenhauer, G., Gschwend, V., Ben-Arie, A., Altevogt, P., & Fogel, M. (2006). Expression profile analysis in multiple human tumors identifies L1 (CD171) as a molecular marker for differential diagnosis and targeted therapy. Human pathology, 37(8), 1000-1008.