Mouse Anti-MAPK1 Recombinant Antibody (ZG-0315J)
CAT#: ZG-0315J
This product is a mouse antibody that recognizes MAPK1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-MAPK1 Recombinant Antibody (ZG-0315J) in WB
Western Blot analysis using ERK 2 Monoclonal Antibody against HeLa (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.
IHC
Figure 2 Mouse Anti-MAPK1 Recombinant Antibody (ZG-0315J) in IHC
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma (left) and breast carcinoma (right) showing cytoplasmic localization with DAB staining using ERK 2 Monoclonal Antibody
IF
Figure 3 Mouse Anti-MAPK1 Recombinant Antibody (ZG-0315J) in IF
mmunofluorescence analysis of Eca-109 cells using ERK 2 Monoclonal Antibody (green).
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79930/1999_H7_3_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal and glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3995/12643_B_9_5_selected.jpg
Subcellular Location
Figure 3 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/21384_B_7_6_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Neuronal cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/21384_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_7_4.jpg
Normal Tissue
Figure 7 Pancreas
Exocrine glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Pancreatic endocrine cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_2_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (K-562)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100030-MAPK1
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Specifications
- Immunogen
- Purified recombinant fragment of human ERK 2 expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MAPK1
- Species Reactivity
- Human, Mouse, Monkey
- Applications
- WB, IHC, IF, ELISA
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- ERK; p38; p40; p41; ERK2; ERT1; NS13; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; mitogen-activated protein kinase 1; MAP kinase 1; MAP kinase 2; MAPK 2; extracellular signal-regulated kinase 2; mitogen-activated protein kinase 2; protein tyrosine kinase ERK2
- Gene ID
- 5594
- UniProt ID
- P28482
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| TAB-0829CL | Anti-Human MAPK1 Recombinant Antibody (VB22B) | ELISA | |
| TAB-0829CL-S(P) | Anti-Human MAPK1 Recombinant Antibody scFv Fragment (VB22B) | ELISA | |
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| CAT | Product Name | Application | Type |
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| BRD-0341MZ | Chicken Anti-MAPK1 Polyclonal IgY | WB | Chicken antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC208 | PE-H-2Kd/Human Erk2 K136Q (QYIHSANVL) MHC Tetramer | FCM | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2148 | Hi-Affi™ Recombinant Rabbit Anti-MAPK1 Monoclonal Antibody (DS2148AB) | WB, IHC-P, ICC, FC, IP | IgG |
| MOR-2155 | Hi-Affi™ Recombinant Rabbit Anti-MAPK3; MAPK1 Monoclonal Antibody (DS2155AB) | WB, IHC-P, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0455U | Rabbit Anti-MAPK1 Recombinant Antibody (clone 10C7) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-QX721 | Mouse Anti-MAPK1 Recombinant Antibody (clone 10C8-9F6-10C8) | IHC | Mouse IgG1 |
| VS3-QX722 | Mouse Anti-MAPK1 Recombinant Antibody (clone 1H4-6D7-9D8) | IHC | Mouse IgG1 |
| VS3-QX723 | Mouse Anti-MAPK1 Recombinant Antibody (clone 5B3-10A5-7B7) | WB, IHC | Mouse IgG1 |
| VS3-QX724 | Mouse Anti-MAPK1 Recombinant Antibody (clone 9A4-2D4-7A3) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1354 | AbPlus™ Anti-MAPK1 Magnetic Beads (VS-0724-YC1354) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY191 | Mouse Anti-NHP MAPK1 Recombinant Antibody (clone 4C11) | WB, IHC, IF, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC691 | CytoStream™ Rabbit Anti-MAPK1 Recombinant Antibody (VS13-YC691) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0525-XY4225 | Anti-Mouse MAPK1 Immunohistochemistry Kit | IHC | |
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