Mouse Anti-MDM2 Recombinant Antibody (VS13-YC717)
CAT#: VS13-YC717
The Mouse Anti-MDM2 Recombinant Antibody is specific for human and monkey MDM2. MDM2 encodes an E3 ubiquitin ligase, promoting tumor formation by targeting tumor suppressors for degradation, like p53. It's involved in various cancers, with amplification or overexpression observed, featuring multiple tumor-specific splice variants. The anti-MDM2 antibody can be used for Western blot (1/1000), Immunofluorescence of cells (1/500), Immunohistochemistry-paraffin (1/1000).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16303/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79977/1999_F7_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human ovary
Immunohistochemical staining of human ovary shows strong nuclear positivity in ovarian stroma cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16303/37938_A_6_7_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79977/170698_A_2_7_selected.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_8_4.jpg
Normal Tissue
Figure 9 Pancreas
Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_2_3.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_8_5.jpg
Normal Tissue
Figure 11 Testis
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_5_6.jpg
Normal Tissue
Figure 12 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Cell line enhanced (HBEC3-KT, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135679-MDM2
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Specifications
- Immunogen
- Recombinant human MDM2, aa 1-491.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human and Monkey MDM2
- Species Reactivity
- Human, Monkey
- Applications
- WB, IHC-P, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 40% Glycerol and 0.1% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000
Immunofluorescence (IF-cell): 1/500
Immunohistochemistry-paraffin (IHC-P): 1/1000
Target
- Alternative Names
- MDM2 Proto-Oncogene; MDM2 Proto-Oncogene, E3 Ubiquitin Protein Ligase; Oncoprotein Mdm2; Hdm2; Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein (Mouse); Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein; Mouse Double Minute 2, Human Homolog Of; P53-Binding Protein; Double Minute 2, Human Homolog Of; P53-Binding Protein; Mdm2, P53 E3 Ubiquitin Protein Ligase Homolog; MDM2 Oncogene, E3 Ubiquitin Protein Ligase;
- Gene ID
- 4193
- UniProt ID
- Q00987
- Long Name
- MDM2 Proto-Oncogene
- Cellular Localization
- Nucleus; Nucleoplasm; Nucleolus; Cytoplasm
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Related Diseases
Bladder Cancer
EGFR Tyrosine Kinase Inhibitor Resistance
Gastric Cancer
Prostate Cancer
Related Signaling Pathways
p53 Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MDM2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-648 | Recombinant Anti-human MDM2 Antibody | ELISA, WB, IF, FuncS | IgG |
| MHH-648 | Recombinant Human Anti-human MDM2 Antibody | IP, RIA, FuncS | IgG |
| VS3-FY917 | Recombinant Mouse Anti-MDM2 Antibody (clone 3C1E3) | ELISA, IHC, FC | Mouse IgG2b |
| VS3-FY918 | Recombinant Mouse Anti-MDM2 Antibody (clone 4A8D12) | ELISA, FC | Mouse IgG1 |
| VS3-FY919 | Recombinant Rabbit Anti-MDM2 (phospho Ser166) Antibody (clone R08-8C7) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-648-F(E) | Recombinant Anti-human MDM2 Antibody Fab Fragment | WB, FACS, FuncS | Fab |
| MHH-648-F(E) | Recombinant Human Anti-human MDM2 Antibody Fab Fragment | ELISA, WB, FC, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-648-S(P) | Recombinant Anti-human MDM2 Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| MHH-648-S(P) | Recombinant Human Anti-human MDM2 Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0353MZ | Chicken Anti-MDM2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4642 | Hi-Affi™ Recombinant Rabbit Anti-MDM2 Monoclonal Antibody (TH155DS) | WB, IF, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1420 | PE-A*02:01/Human MDM2 (LLGDLFGV) MHC Tetramer | FCM | |
| MHC-YF287 | A*02:01/Human MDM2 (LLGDLFGV) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4322 | Anti-MDM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4323 | Anti-Human MDM2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-FY8 | Mouse Anti-MDM2 scFv-Fc Chimera (VS-0625-FY8) | ELISA, WB | Mouse IgG1, scFv-Fc |
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