Rabbit Anti-MMP14 Recombinant Antibody (VS3-CJ1006)
CAT#: VS3-CJ1006
This product is a rabbit antibody that recognizes human, mouse, and rat MMP14.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51432/1591_G3_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong luminal membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51432/134761_A_8_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows positivity in stromal cells and plasma.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51432/1658_G1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC diff, HHSteC, HSkMC, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000157227-MMP14
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat MMP14
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
- Gene ID
- 4323
- UniProt ID
- P50281
- Sequence Similarities
- Belongs to the peptidase M10A family.
- Cellular Localization
- Cytoplasm, Membrane
- Post Translation Modifications
- The precursor is cleaved by a furin endopeptidase.
Tyrosine phosphorylated by PKDCC/VLK.
- Protein Refseq
- NP_004986.1
- Function
- Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity).
May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed:20837484).
Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed:12714657).
Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed:22330140).
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MMP14"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-865-VHH | Recombinant Anti-human MMP14 VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-665-S(P) | Recombinant Anti-human MMP14 Antibody scFv Fragment | FC, Neut, Funcs | scFv |
| MHH-665-S(P) | Recombinant Human Anti-human MMP14 Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| HPAB-0260CQ-S(P) | Human Anti-MMP14 Recombinant Antibody; scFv Fragment (HPAB-0260CQ-S(P)) | ELISA, FC, IHC | Human scFv |
| HPAB-0623-WJ-S(P) | Human Anti-MMP14 Recombinant Antibody; scFv Fragment (HPAB-0623-WJ-S(P)) | ELISA | Human scFv |
| HPAB-0624-WJ-S(P) | Human Anti-MMP14 Recombinant Antibody; scFv Fragment (HPAB-0624-WJ-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0822CL | Human Anti-MMP14 Recombinant Antibody (TAB-0822CL) | ELISA, FuncS, FC, IHC | Human IgG |
| TAB-0823CL | Human Anti-MMP14 Recombinant Antibody (TAB-0823CL) | ELISA | Human IgG |
| TAB-0822CL-S(P) | Human Anti-MMP14 Recombinant Antibody; scFv Fragment (TAB-0822CL-S(P)) | ELISA, FC, IHC | Human scFv |
| TAB-0822CL-F(E) | Human Anti-MMP14 Recombinant Antibody; Fab Fragment (TAB-0822CL-F(E)) | ELISA, FC, IHC | Human Fab |
| TAB-0823CL-F(E) | Human Anti-MMP14 Recombinant Antibody; Fab Fragment (TAB-0823CL-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1237CT | Recombinant Mouse anti-Human MMP14 Monoclonal antibody (EML768) | IHC-P, WB | |
| HPAB-0623-WJ | Human Anti-MMP14 Recombinant Antibody (HPAB-0623-WJ) | ELISA | Human IgG |
| HPAB-0624-WJ | Human Anti-MMP14 Recombinant Antibody (HPAB-0624-WJ) | ELISA | Human IgG |
| HPAB-0625-WJ | Human Anti-MMP14 Recombinant Antibody (HPAB-0625-WJ) | ELISA | Human IgG |
| HPAB-0626-WJ | Human Anti-MMP14 Recombinant Antibody (HPAB-0626-WJ) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0370MZ | Chicken Anti-MMP14 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2251 | Hi-Affi™ Recombinant Rabbit Anti-MMP14 Monoclonal Antibody (DS2251AB) | IHC-P, ICC, IF, FC, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0260CQ-F(E) | Human Anti-MMP14 Recombinant Antibody; Fab Fragment (HPAB-0260CQ-F(E)) | ELISA, FC, IHC | Human Fab |
| HPAB-0623-WJ-F(E) | Human Anti-MMP14 Recombinant Antibody; Fab Fragment (HPAB-0623-WJ-F(E)) | ELISA | Human Fab |
| HPAB-0624-WJ-F(E) | Human Anti-MMP14 Recombinant Antibody; Fab Fragment (HPAB-0624-WJ-F(E)) | ELISA | Human Fab |
| HPAB-0625-WJ-F(E) | Human Anti-MMP14 Recombinant Antibody; Fab Fragment (HPAB-0625-WJ-F(E)) | ELISA | Human Fab |
| HPAB-0626-WJ-F(E) | Human Anti-MMP14 Recombinant Antibody; Fab Fragment (HPAB-0626-WJ-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ1005 | Mouse Anti-MMP14 (C-Terminus) Recombinant Antibody (VS3-CJ1005) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC295 | Recombinant Anti-MMP14 Vesicular Antibody, EV Displayed (VS-0425-YC295) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4447 | Anti-MMP14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4448 | Anti-Mouse MMP14 Immunohistochemistry Kit | IHC |
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