Rabbit Anti-SELPLG Recombinant Antibody (clone R03-8N8)

Name: Rabbit Anti-SELPLG Recombinant Antibody (clone R03-8N8)
SKU: VS3-XY3498
Rating: 5 (1 reviews)

CAT#: VS3-XY3498

This is a recombinant rabbit antibody that specifically recognizes human SELPLG (Selectin P ligand), which is critical for the initial leukocyte capture. The antibody also has cross-reactivity against mouse or rat SELPLG. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HEL

Immunofluorescent staining of human cell line HEL shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11256/1847_F9_37_cr5abb5d8e30c09_selected.jpg

Normal Tissue Figure 2 High expression in spleen.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2431/6181_B_8_4_val_selected.jpg

Normal Tissue Figure 3 Kidney

Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69062/170043_A_9_5.jpg

Normal Tissue Figure 4 Lymph node

Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69062/170043_A_9_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (HDLM-2, HMC-1, JURKAT, Karpas-707, RPMI-8226, U-266/70, U-266/84)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110876-SELPLG

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Specifications

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human SELPLG

Species Reactivity
Human

Clone
R03-8N8

Applications
WB, IHC-P, ICC, IF, IP

Related Disease
Hand, Foot And Mouth Disease; Human Granulocytic Anaplasmosis

Product Property

Clonality
Monoclonal

Aggregation
< 5%

Format
Liquid

Buffer
PBS (pH 7.4), 150 mM NaCl and 50% glycerol

Preservative
0.02% sodium azide

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20

Target

Alternative Names
CLA; CD162; PSGL1; PSGL-1

Gene ID
6404

UniProt ID
Q14242

Long Name
Selectin P ligand

Cellular Localization
Membrane

Post Translation Modifications
Glycosylation at Thr57, Asn65, Asn111, Ser287, Ser289, Ser290, Thr292, and Asn302 (NX_Q14242 [NX_Q14242-1])
Ubiquitination at Lys386 (NX_Q14242 [NX_Q14242-1])
Modification sites at PhosphoSitePlus (Q14242)
Glycosylation from GlyConnect SELPL_HUMAN (520)
Glycosylation from GlyGen (Q14242) 12 sites, 10 O-linked glycans (8 sites)

Protein Refseq
NP_002997.2

Function
A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
Critical for the initial leukocyte capture. SELPL_HUMAN,Q14242
(Microbial infection) Acts as a receptor for enterovirus 71.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-XY3498.pdf

MSDS

MSDS - VS3-XY3498.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

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Recommended Dilution Buffer

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Secondary Antibody

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