Anti-TEK Antibody Prodrug, Protease Activated (15B8)

Name: Anti-TEK Antibody Prodrug, Protease Activated (15B8)
SKU: VS-1025-YC97
Rating: 5 (1 reviews)

CAT#: VS-1025-YC97

The Anti-TEK Antibody Prodrug, Protease Activated (15B8) is an antibody prodrug comprised of a recombinant mouse antibody targeting human TEK, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to TEK protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to TEK effectively. TEK, or TIE2, is a receptor tyrosine kinase expressed almost exclusively on endothelial cells, playing a key role in the stabilization and maturation of new blood vessels. Signaling through TIE2 in the tumor microenvironment is essential for maintaining tumor angiogenesis and is associated with tumor progression and metastasis.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & centriolar satellites.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1607_D9_2_selected.jpg

Subcellular Location Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/ihc_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line AF22

Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane & centriolar satellites.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1687_H4_1_red_green.jpg

Subcellular Location Figure 4 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane & centriolar satellites.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1667_D9_1_red_green.jpg

Normal Tissue Figure 5 Colon

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/24108_A_8_3.jpg

Normal Tissue Figure 6 Testis

Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/24108_A_6_6.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (HUVEC TERT2, NTERA-2, TIME)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 7 RNA cell line category: Group enriched (HUVEC TERT2, NTERA-2, TIME)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120156-TEK

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Specifications

Host Animal
Mouse

Applications
In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay

Specificity
Human TEK

Target
TEK

Species Reactivity
Human

Isotype
IgG

Clone
15B8

Epitope
TEK-ECD

Affinity Constant
K_D = 0.24 nM

Masking Approach
Affinity mask

Mask
A synthetic peptide

Protease Cleavable Linker
MMP-cleavable linker

Protease Substrate
MMPs

Purification
Protein A purified

Buffer
PBS, pH 7.4

Preservative
No preservatives

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target

Introduction
This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Alternative Names
TIE2; VMCM; TIE-2; VMCM1; CD202B

Gene ID
7010

UniProt ID
Q02763

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Datasheet

Datasheet - VS-1025-YC97.pdf

MSDS

MSDS - VS-1025-YC97.pdf

COA

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