Recombinant Anti-human TEK Intrabody [+36 GFP]
CAT#: IAB-B064(G)
Recombinant anti-TEK intrabody in fusion with +36GFP works within cells to bind human TEK, expressed in E. coli cells.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1607_D9_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1687_H4_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to plasma membrane & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1667_D9_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/24108_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/24108_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (HUVEC TERT2, NTERA-2, TIME)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 7 RNA cell line category: Group enriched (HUVEC TERT2, NTERA-2, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120156-TEK
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Specifications
- Immunogen
- human TEK tyrosine kinase, endothelial
- Host Species
- human
- Type
- scFv-(+36GFP)
- Cell Penetrating Peptide
- +36 GFP
GFP with a theoretical net charge of +36 (+36 GFP) penetrated several different mammalian cell lines at very low (nanomolar) concentrations. Cationic peptides and proteins have been observed to penetrate cells, and both natural and synthetic cell-penetrating peptides are widely used as delivery vehicles. However, whereas a covalent link between the cell-penetrating peptide and its cargo is typically required.
- Species Reactivity
- human
- Applications
- Antibody assay: Western blot; Neutralization; Functional studies
Product Property
- Purity
- >90% assessed by SDS-PAGE
- Storage
- ≥1 year at -20°C. If the reconstituted antibody cannot be used within two weeks, it should be aliquoted into smaller vials and stored at -20°C
Target
- Alternative Names
- TEK; TEK tyrosine kinase, endothelial; TIE2; VMCM; TIE-2; VMCM1; CD202B; angiopoietin-1 receptor; endothelial tyrosine kinase; tyrosine-protein kinase receptor TEK; tunica interna endothelial cell kinase; tyrosine-protein kinase receptor TIE-2; tyrosine k
- Gene ID
- 7010
- UniProt ID
- Q02763
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| HPAB-S0140-YC-S(P) | Mouse Anti-TEK Recombinant Antibody (clone 6A3); scFv Fragment | ELISA, Antagonist | Mouse scFv |
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