Mouse Anti-TEK (clone 12H8) scFv-Fc Chimera (CAT#: VS-0325-FY76)
The anti-TEK scFv-Fc antibody targets TEK (TIE2), a receptor tyrosine kinase involved in angiogenesis and vascular development. It can modulate TEK signaling pathways, making it a potential tool for studying vascular biology and related diseases. The scFv-Fc format enhances its stability and bioavailability, allowing for prolonged action in vivo.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1607_D9_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1607_D9_2_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/ihc_selected.jpg
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/ihc_selected.jpg
Figure 3 IF staining of human cell line AF22
(Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1687_H4_1_red_green.jpg
(Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1687_H4_1_red_green.jpg
Figure 4 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1667_D9_1_red_green.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73265/1667_D9_1_red_green.jpg
Figure 5 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/24108_A_8_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/24108_A_8_3.jpg
Figure 6 Testis
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/24108_A_6_6.jpg
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10359/24108_A_6_6.jpg
Figure 7 RNA cell line category: Group enriched (HUVEC TERT2, NTERA-2, TIME)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000120156-TEK
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000120156-TEK
Figure 7 RNA cell line category: Group enriched (HUVEC TERT2, NTERA-2, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120156-TEK
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120156-TEK
Specifications
- Immunogen
- Recombinant Tie2 extracellular domain-Fc fusion
- Host Species
- Mouse
- Type
- Mouse IgG1, scFv-Fc
- Specificity
- Human TEK
- Species Reactivity
- Human
- Clone
- 12H8
- Applications
- Antagonist
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- TEK Receptor Tyrosine Kinase; Tyrosine Kinase With Ig And EGF Homology Domains-2; Tunica Interna Endothelial Cell Kinase; Tyrosine-Protein Kinase Receptor TIE-2; Tyrosine-Protein Kinase Receptor TEK; TEK Tyrosine Kinase, Endothelial; Endothelial Tyrosine Kinase; EC 2.7.10.1; VMCM1; VMCM; TIE2
- Gene ID
- 7010
- UniProt ID
- Q02763
- Long Name
- TEK Receptor Tyrosine Kinase
- Sequence Similarities
- Belongs to the protein kinase superfamily.
- Cellular Localization
- Cell membrane; Cell junction; Cell junction, focal adhesion; Cytoplasm, cytoskeleton; Secreted
- Post Translation Modifications
- Ubiquitinated.
The phosphorylated receptor is ubiquitinated and internalized, leading to its degradation.
Glycosylation at Asn140, Asn158, Asn399, Asn438, Asn464, Asn560, Asn596, Asn649, Asn691, and Asn1104
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect TIE2_HUMAN (769)
Glycosylation from GlyGen 10 sites, 12 N-linked glycans (6 sites)
- Protein Refseq
- NP_000450.3; NP_001277006.2; NP_001277007.2; NP_001362404.1; NP_001362405.1
- Function
- TEK (Tyrosine kinase, endothelial) also known as Tie2, is a receptor tyrosine kinase that plays a crucial role in angiogenesis and vascular development. It is essential for blood vessel formation and maintenance.
Recommended Products
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1030 | Mouse Anti-TEK Recombinant Antibody (clone YC183); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0066-YJ-F(E) | Recombinant Mouse Anti-TEK Antibody Fab Fragment (12H8) | Immunoassay, WB | Mouse Fab |
| HPAB-J0235-YC-F(E) | Human Anti-TEK Recombinant Antibody; Fab Fragment (HPAB-J0235-YC-F(E)) | ELISA, Inhib, FuncS | Human Fab |
| HPAB-S0139-YC-F(E) | Mouse Anti-TEK Recombinant Antibody (clone 12H8); Fab Fragment | ELISA, Antagonist | Mouse Fab |
| HPAB-S0140-YC-F(E) | Mouse Anti-TEK Recombinant Antibody (clone 6A3); Fab Fragment | ELISA, Antagonist | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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