Human Anti-STAT3 scFv-Fc Chimera (VS-0425-FY28)
CAT#: VS-0425-FY28
The anti-STAT3 scFv-Fc antibody is a recombinant human antibody that specifically recognizes STAT3. STAT3 is an important signaling transducer involved in various biological processes such as cell proliferation, differentiation, and apoptosis. This antibody specifically binds to STAT3 through its single-chain variable fragment (scFv) domain, while the Fc fragment endows it with the ability to interact with immune effector molecules such as Fc receptors and the complement system.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1671/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong nuclear positivity and cytoplasmic in decidual cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1671/155480_A_3_7_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58603/ihc_selected.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68241/153085_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_7_5.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_8_8.jpg
Normal Tissue
Figure 7 Lung
Alveolar cells type I Staining: Medium Intensity: Moderate Quantity: 75%-25% Alveolar cells type I Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Macrophages Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_3_4.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68242/153074_A_7_3.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168610-STAT3
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human STAT3
- Species Reactivity
- Human
- Applications
- ELISA, Immunofluorescence
- Related Disease
- Hyper-Ige Syndrome 1, Autosomal Dominant, With Recurrent Infections and Autoimmune Disease, Multisystem, Infantile-Onset, 1
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 20 ug/mL
Immunofluorescence: 10 ug/mL
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Signal transducer and activator of transcription 3; Stat3
- Long Name
- Signal transducer and activator of transcription 3
- Sequence Similarities
- Belongs to the transcription factor STAT family.
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Tyrosine phosphorylated upon stimulation with EGF.
Acetylated on lysine residues by EP300/p300, promoting its activation.
Some lysine residues are oxidized to allysine by LOXL3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity.
Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated.
(Microbial infection) Phosphorylated on Tyr-705 in the presence of S.typhimurium SarA.
Ubiquitination at Lys87, Lys97, Lys177, Lys180, Lys244, Lys294, and Lys709
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 6 sites, 2 O-linked glycans (6 sites)
- Protein Refseq
- XP_016880462.1; XP_047292541.1; XP_047292542.1; XP_054172966.1; XP_054172967.1
- Function
- Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors.
Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene.
May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes.
Activated by IL31 through IL31RA
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Related Diseases
Endometrial Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Throid Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Related Signaling Pathways
MAPK Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOR-4711 | Hi-Affi™ Recombinant Rabbit Anti-STAT3 Monoclonal Antibody (TH225DS) | WB, ELISA | IgG |
| MOR-0051-FY | Rabbit Anti-STAT3 Recombinant Antibody (clone AFY0022) | ICC, IHC-P, WB | Rabbit IgG |
| MOR-0053-FY | Rabbit Anti-STAT3 Recombinant Antibody (clone AFY0024) | IHC-P, WB, Inhib | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2385-CN | Recombinant Rabbit Anti-STAT3 (phosphorylated Ser727) Monoclonal Antibody (CBACN-670) | WB, IF, IHC, IP | Rabbit IgG |
| MRO-2386-CN | Recombinant Rabbit Anti-STAT3 (phosphorylated Thryr705) Monoclonal Antibody (CBACN-671) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-114R | Mouse Anti-STAT3 Recombinant Antibody (ZG-114R) | WB, ELISA, IHC | Mouse IgG |
| ZG-115R | Mouse Anti-STAT3 Recombinant Antibody (ZG-115R) | WB, ELISA, IHC, IF | Mouse IgG |
| ZG-116R | Mouse Anti-STAT3 Recombinant Antibody (ZG-116R) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP506-YC | Camel Anti-STAT3 Recombinant Antibody (HPAB-AP506-YC) | ELISA | Camel VHH |
| HPAB-AP507-YC | Camel Anti-STAT3 Recombinant Antibody (HPAB-AP507-YC) | ELISA | Camel VHH |
| HPAB-AP508-YC | Camel Anti-STAT3 Recombinant Antibody (HPAB-AP508-YC) | ELISA | Camel VHH |
| HPAB-AP509-YC | Camel Anti-STAT3 Recombinant Antibody (HPAB-AP509-YC) | ELISA, IP, Inhib, Cyt, FuncS | Camel VHH |
| HPAB-AP510-YC | Camel Anti-STAT3 Recombinant Antibody (HPAB-AP510-YC) | ELISA, IP, Inhib, Cyt, FuncS | Camel VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY249 | AbPlus™ Anti-STAT3 Magnetic Beads (CBACN-530) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-0325-XY2195 | Anti-STAT3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6951 | Anti-Mouse STAT3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6950 | Anti-Human STAT3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6953 | Anti-Zebrafish STAT3 Immunohistochemistry Kit | IHC |
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