Recombinant Rabbit Anti-BAD Antibody (clone R07-1B6)
CAT#: VS3-FY131
This product is a recombinant rabbit antibody that recognizes human, mouse and rat BAD protein. It can detect a band of approximately 18 kDa (observedd molecular weight: 23 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of cells in germinal and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62105/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4205/11845_A_9_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4205/11825_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000002330-BAD
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WB
Figure 1. Recombinant Rabbit Anti-BAD Antibody (clone R07-1B6) in WB.
Western blot analysis of Bad in rat brain, C6, 3T3, Hela lysates using Bad Antibody.
IHC
Figure 2. Recombinant Rabbit Anti-BAD Antibody (clone R07-1B6) in IHC.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Bad antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specifications
- Immunogen
- A synthetic peptide of human Bad
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BAD
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-1B6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 18 kDa; Observed MW: 23 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Related Signaling Pathways
cAMP Signaling Pathway
ErbB Signaling Pathway
PI3K-Akt Signaling Pathway
Related Diseases
Colorectal Cancer
Endometrial Cancer
Amyotrophic Lateral Sclerosis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "BAD"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0064MZ | Chicken Anti-BAD Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0317 | Hi-Affi™ Rabbit Anti-BAD Recombinant Antibody (clone DS317AB) | IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0149-CN | Rabbit Anti-BAD Recombinant Antibody (clone CBACN-058) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-1677-CN | Rabbit Anti-BAD Polyclonal Antibody (MRO-1677-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-XY115 | Mouse Anti-BAD Recombinant Antibody (clone 1B1) | ELISA, WB, FC | Mouse IgG1 |
| VS3-WK1090 | Rabbit Anti-BAD Recombinant Antibody (VS3-WK1090) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-WK2175 | Rabbit Anti-BAD (pS112) Recombinant Antibody (clone R07-7Y9) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY1 | Mouse Anti-BAD Agonistic Antibody (VS-0724-XY1) | WB, IHC, ICC | Mouse IgG2a |
| VS-0724-XY2 | Rat Anti-Bad Agonistic Antibody (VS-0724-XY2) | WB, ICC | Rat IgG2a |
| VS-0724-XY3 | Mouse Anti-BAD Agonistic Antibody (VS-0724-XY3) | ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY46 | Rabbit Anti-NHP BAD Recombinant Antibody (VS-1024-XY46) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC83 | CytoStream™ Rabbit Anti-BAD Recombinant Antibody (VS13-YC83) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY680 | Anti-BAD Immunohistochemistry Kit | IHC |
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