Recombinant Anti-CD40LG Vesicular Antibody, EV Displayed (VS-0425-YC389)

Name: Recombinant Anti-CD40LG Vesicular Antibody, EV Displayed (VS-0425-YC389)
SKU: VS-0425-YC389
Rating: 5 (1 reviews)

CAT#: VS-0425-YC389

The Recombinant Anti-CD40LG Vesicular Antibody, EV Displayed (VS-0425-YC389) is an antibody-displaying extracellular vesicle (Ab-EV). The product combines the benefits of both extracellular vesicle (EV) and antibody (Ab) which can guide the decorated EVs to CD40LG-expressed cells or tissues. The CD40LG is CD40 ligand, involved in immune signaling pathways, with mutations associated with immunodeficiency disorders.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line JURKAT

Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2010_G3_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line HEL

Immunofluorescent staining of human cell line HEL shows localization to golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2069_B6_2_blue_green.jpg

Normal Tissue Figure 3 Colon

Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_3.jpg

Normal Tissue Figure 4 Lymph node

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enriched (MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG

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Recombinant Antibody

Application
ELISA, FC, Neut, Cell-uptake

Product Type
Ab-Fc-EVs

Antibody Quantification (Ab/EV)
~100 Ab/EV

Target
CD40LG

Host Animal
Human

Antibody Isotype
IgG

Species Reactivity
Human

Expression Cell
Mammalian cell

Engineered EVs

EV-sorting domain
CD63

Fc-binding domain
z domain

EV Size
30~150 nm

Producing Cell
HEK293F

Isolation Method
Gradient centrifugation

Purification
qEV size exclusion chromatography

Binding Affinity
Kd = 0.85 µg/mL

Concentration
1 x 10¹⁰

Size
1 mL

Buffer
PBS

Storage
Store at -80°C for 12 months

Target

Full Name
CD40 ligand

Molecular Function
Cytokine

Cellular Localization
Golgi apparatus, Plasma membrane

Introduction
CD40LG (CD40 Ligand) is a Protein Coding gene. Diseases associated with CD40LG include Immunodeficiency With Hyper-Igm, Type 1 and Cd40 Ligand Deficiency. Among its related pathways are PEDF Induced Signaling and Cytokine Signaling in Immune system. Gene Ontology (GO) annotations related to this gene include cytokine activity and CD40 receptor binding.

Alternative Names
CD154, CD40-L, CD40L, gp39, hCD40L, HIGM1, IMD3, T-BAM, TNFSF5, TRAP

Gene ID
959

UniProt ID
P29965

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Downloadable Resources

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Datasheet

Datasheet - VS-0425-YC389.pdf

MSDS

MSDS - VS-0425-YC389.pdf

COA

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