CytoStream™ Rabbit Anti-CD40LG Recombinant Antibody (VS13-YC164)
CAT#: VS13-YC164
The CytoStream™ Rabbit Anti-CD40LG Recombinant Antibody is specific for human, mouse and rat CD40LG. CD40LG involves ligand roles in immune system pathways affecting signaling mechanisms in immune deficiencies by modulating receptor-binding functions. The anti-CD40LG antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2010_G3_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2069_B6_2_blue_green.jpg
Normal Tissue
Figure 3 Colon
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_3.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG
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Specifications
- Immunogen
- Recombinant human CD40L, aa 103-150.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CD40LG
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
- Related Disease
- Immunodeficiency With Hyper-Igm, Type 1 and Cd40 Ligand Deficiency
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- CD40 Ligand; TNF-Related Activation Protein; Tumor Necrosis Factor (Ligand) Superfamily Member 5; T-B Cell-Activating Molecule; CD40 Antigen Ligand; T-Cell Antigen Gp39; TNFSF5; CD40-L; CD40L; TRAP; Tumor Necrosis Factor (Ligand) Superfamily, Member 5 (Hyper-IgM Syndrome);
- Gene ID
- 959
- UniProt ID
- P29965
- Long Name
- CD40 Ligand
- Cellular Localization
- Secreted; Cell membrane; Cell surface
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CD40LG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1071z | Mouse Anti-CD40LG Recombinant Antibody (clone 18C1) | ELISA, FC, FuncS, ICC, IF | Mouse IgG1 |
| PABW-020 | Human Anti-CD40LG Recombinant Antibody (PABW-020) | Neut | Human IgG1, κ |
| FAMAB-0135YC | Hamster Anti-CD40LG Recombinant Antibody (clone mr-1) | ELISA | Hamster IgG |
| HPAB-0880LY | Mouse Anti-CD40LG Recombinant Antibody (HPAB-0880LY) | FC | Mouse IgG1 |
| HPAB-0881LY | Mouse Anti-CD40LG Recombinant Antibody (HPAB-0881LY) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-027 | Anti-Human CD40L Recombinant Antibody (TAB-027) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
| TAB-876 | Anti-Human CD40 ligand Recombinant Antibody (Toralizumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
| TAB-211LC | Human Anti-CD40LG Recombinant Antibody (TAB-211LC) | ELISA | Human IgG, κ |
| TAB-210LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-210LC-F(E)) | ELISA | Humanized Fab |
| TAB-211LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-211LC-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G080E | Anti-Cd40lg immunotoxin LL2 (IgG)-PE | Cytotoxicity assay, Function study | |
| AGTO-G081E | Anti-Cd40lg immunotoxin LL2 (Fab)-PE | Cytotoxicity assay, Function study | |
| AGTO-L051P | anti-CD40LG immunotoxin MR1 (scFv)-PAP | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-020 | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (PSBW-020) | Neut | Human scFv |
| FAMAB-0135-YC-S(P) | Hamster Anti-CD40LG Recombinant Antibody (clone mr-1); scFv Fragment | ELISA | Hamster scFv |
| HPAB-0880LY-S(P) | Mouse Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0880LY-S(P)) | FC | Mouse scFv |
| HPAB-0881LY-S(P) | Mouse Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0881LY-S(P)) | FC | Mouse scFv |
| HPAB-0882LY-S(P) | Mouse Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0882LY-S(P)) | FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-212LC | Mouse Anti-Human CD40LG Recombinant Antibody (ABI793) | Block | |
| TAB-212LC-S(P) | Anti-Human CD40LG Recombinant Antibody scFv Fragment (ABI793) | ELISA | |
| TAB-212LC-F(E) | Anti-Human CD40LG Recombinant Antibody Fab Fragment (ABI793) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-225LC | Anti-Human CD40LG Recombinant Antibody (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
| TAB-225LC-S(P) | Anti-Human CD40LG Recombinant Antibody scFv Fragment (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
| TAB-225LC-F(E) | Anti-Human CD40LG Recombinant Antibody Fab Fragment (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-213LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-213LC-F(E)) | FC, ELISA, RBA | |
| TAB-214LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-214LC-F(E)) | FC, ELISA, RBA | |
| TAB-215LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-215LC-F(E)) | FC, ELISA, RBA | |
| TAB-216LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-216LC-F(E)) | FC, ELISA, RBA | |
| TAB-217LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-217LC-F(E)) | FC, ELISA, RBA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-368CQ | Mouse Anti-CD40LG Recombinant Antibody (clone 24-31) | FC, Block, IF | Mouse IgG1, κ |
| NEUT-375CQ | Hamster Anti-CD40LG Recombinant Antibody (clone MR1) | IHC-Fr, FC, IP, Block | Hamster IgG1, κ |
| NEUT-377CQ | Hamster Anti-CD40LG Recombinant Antibody (clone MAB0820) | Block, FC, IHC-Fr | Hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-369CQ | Mouse Anti-CD40LG Recombinant Antibody (clone CBL300) | FC, IHC, CyTOF, Neut, WB | Mouse IgG2b |
| NEUT-370CQ | Mouse Anti-CD40LG Recombinant Antibody (clone LL2) | Neut, ELISA, FC, FuncS, IF | Mouse IgG1 |
| NEUT-371CQ | Mouse Anti-CD40LG Recombinant Antibody (clone LL48) | Neut, FuncS, IF | Mouse IgG2a |
| NEUT-372CQ | Mouse Anti-CD40LG Recombinant Antibody (NEUT-372CQ) | Neut | Mouse IgG2b |
| NEUT-374CQ | Rat Anti-CD40LG Recombinant Antibody (clone CBL719) | FC, CyTOF, Neut | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0561 | Hi-Affi™ Rabbit Anti-CD40LG Recombinant Antibody (clone DS561AB) | WB, FC | Rabbit IgG |
| MOR-0562 | Hi-Affi™ Rabbit Anti-CD40LG Recombinant Antibody (clone DS562AB) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0610LC | Mouse Anti-CD40LG Recombinant Antibody (clone h5c8) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0179-YC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0179-YC-F(E)) | ELISA | Human Fab |
| HPAB-0880LY-F(E) | Mouse Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0880LY-F(E)) | FC | Mouse Fab |
| HPAB-0881LY-F(E) | Mouse Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0881LY-F(E)) | FC | Mouse Fab |
| HPAB-0882LY-F(E) | Mouse Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0882LY-F(E)) | FC | Mouse Fab |
| HPAB-0585-WJ-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0585-WJ-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-022CN | Human Anti-CD40LG Recombinant Antibody (NS-022CN) | ELISA, FC | Human IgG |
| NS-022CN-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (NS-022CN-F(E)) | ELISA, FC | Human Fab |
| NS-022CN-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (NS-022CN-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-027 | Afuco™ Anti-CD40LG ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-027) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-876 | Afuco™ Anti-CD40LG ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-876) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY79 | Human Anti-CD40LG (clone YMF323.6) scFv-Fc Chimera | FC, ELISA, IHC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC389 | Recombinant Anti-CD40LG Vesicular Antibody, EV Displayed (VS-0425-YC389) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1182 | Anti-CD40LG Immunohistochemistry Kit | IHC | |
| VS-0525-XY1183 | Anti-Mouse CD40LG Immunohistochemistry Kit | IHC |
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