Anti-Cd40lg immunotoxin LL2 (Fab)-PE (CAT#: AGTO-G081E)
This immunotoxin was achieved by conjugating/fusing the Anti-Cd40lg Fab to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating B-cell lymphoma.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line JURKAT
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2010_G3_1_selected.jpg
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2010_G3_1_selected.jpg
Figure 2 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2069_B6_2_blue_green.jpg
(Immunofluorescent staining of human cell line HEL shows localization to golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2069_B6_2_blue_green.jpg
Figure 3 Colon
(Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_3.jpg
(Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_3.jpg
Figure 4 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_8.jpg
Figure 5 RNA cell line category: Cell line enriched (MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG
Figure 5 RNA cell line category: Cell line enriched (MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG
Specifications
- Host Species
- Hamster
- Species Reactivity
- Mouse
- Clone
- LL2
- Molecule Class
- Fab-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- B-cell lymphoma
Target
- Alternative Names
- IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Cd40lg; CD40 ligand; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE105
- Gene ID
- 21947
- UniProt ID
- P27548
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone LL2"
See other products for "CD40LG"
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-027 | Anti-Human CD40L Recombinant Antibody (Ruplizumab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
| TAB-876 | Anti-Human CD40 ligand Recombinant Antibody (Toralizumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
| TAB-211LC | Human Anti-CD40LG Recombinant Antibody (TAB-211LC) | ELISA | Human IgG, κ |
| TAB-210LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-210LC-F(E)) | ELISA | Humanized Fab |
| TAB-211LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-211LC-F(E)) | ELISA | Humanized Fab |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G080E | Anti-Cd40lg immunotoxin LL2 (IgG)-PE | Cytotoxicity assay, Function study | |
| AGTO-L051P | anti-CD40LG immunotoxin MR1 (scFv)-PAP | Cytotoxicity assay, Functional assay |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-020 | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (PFBW-020) | Neut | Human Fab |
| HPAB-0179-YC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0179-YC-F(E)) | ELISA | Human Fab |
| FAMAB-0135-YC-F(E) | Hamster Anti-CD40LG Recombinant Antibody (clone mr-1); Fab Fragment | ELISA | Hamster Fab |
| HPAB-0744LY-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0744LY-F(E)) | ELISA | Human Fab |
| HPAB-0585-WJ-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0585-WJ-F(E)) | ELISA | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-212LC | Mouse Anti-Human CD40LG Recombinant Antibody (ABI793) | Block | |
| TAB-212LC-S(P) | Anti-Human CD40LG Recombinant Antibody scFv Fragment (ABI793) | ELISA | |
| TAB-212LC-F(E) | Anti-Human CD40LG Recombinant Antibody Fab Fragment (ABI793) | ELISA |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-225LC | Anti-Human CD40LG Recombinant Antibody (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
| TAB-225LC-S(P) | Anti-Human CD40LG Recombinant Antibody scFv Fragment (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
| TAB-225LC-F(E) | Anti-Human CD40LG Recombinant Antibody Fab Fragment (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-213LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-213LC-S(P)) | FC, ELISA, RBA | |
| TAB-214LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-214LC-S(P)) | FC, ELISA, RBA | |
| TAB-215LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-215LC-S(P)) | FC, ELISA, RBA | |
| TAB-216LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-216LC-S(P)) | FC, ELISA, RBA | |
| TAB-217LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-217LC-S(P)) | FC, ELISA, RBA |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-368CQ | Mouse Anti-CD40LG Recombinant Antibody (clone 24-31) | FC, Block, IF | Mouse IgG1, κ |
| NEUT-375CQ | Hamster Anti-CD40LG Recombinant Antibody (clone MR1) | IHC-Fr, FC, IP, Block | Hamster IgG1, κ |
| NEUT-377CQ | Hamster Anti-CD40LG Recombinant Antibody (clone MAB0820) | Block, FC, IHC-Fr | Hamster IgG |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-369CQ | Mouse Anti-CD40LG Recombinant Antibody (clone CBL300) | FC, IHC, CyTOF, Neut, WB | Mouse IgG2b |
| NEUT-370CQ | Mouse Anti-CD40LG Recombinant Antibody (clone LL2) | Neut, ELISA, FC, FuncS, IF | Mouse IgG1 |
| NEUT-371CQ | Mouse Anti-CD40LG Recombinant Antibody (clone LL48) | Neut, FuncS, IF | Mouse IgG2a |
| NEUT-372CQ | Mouse Anti-CD40LG Recombinant Antibody (clone 13H5) | Neut | Mouse IgG2b |
| NEUT-374CQ | Rat Anti-CD40LG Recombinant Antibody (clone CBL719) | FC, CyTOF, Neut | Rat IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0561 | Hi-Affi™ Rabbit Anti-CD40LG Recombinant Antibody (clone DS561AB) | WB, FC | Rabbit IgG |
| MOR-0562 | Hi-Affi™ Rabbit Anti-CD40LG Recombinant Antibody (clone DS562AB) | ELISA | Rabbit IgG |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0610LC | Mouse Anti-CD40LG Recombinant Antibody (clone h5c8) | ELISA | Mouse IgG1 |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0179-YC | Human Anti-CD40LG Recombinant Antibody (HPAB-0179-YC) | ELISA | Human IgG1 |
| HPAB-0880LY | Mouse Anti-CD40LG Recombinant Antibody (HPAB-0880LY) | FC | Mouse IgG1 |
| HPAB-0881LY | Mouse Anti-CD40LG Recombinant Antibody (HPAB-0881LY) | FC | Mouse IgG1 |
| HPAB-0882LY | Mouse Anti-CD40LG Recombinant Antibody (HPAB-0882LY) | FC | Mouse IgG1 |
| MRO-0304-CN | Rabbit Anti-CD40LG Recombinant Antibody (clone JM11-34) | WB, IF, IHC, FC | Rabbit IgG |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-022CN | Human Anti-CD40LG Recombinant Antibody (NS-022CN) | ELISA, FC | Human IgG |
| NS-022CN-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (NS-022CN-F(E)) | ELISA, FC | Human Fab |
| NS-022CN-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (NS-022CN-S(P)) | ELISA, FC | Human scFv |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0135-YC-S(P) | Hamster Anti-CD40LG Recombinant Antibody (clone mr-1); scFv Fragment | ELISA | Hamster scFv |
| HPAB-0880LY-S(P) | Mouse Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0880LY-S(P)) | FC | Mouse scFv |
| HPAB-0881LY-S(P) | Mouse Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0881LY-S(P)) | FC | Mouse scFv |
| HPAB-0882LY-S(P) | Mouse Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0882LY-S(P)) | FC | Mouse scFv |
| HPAB-0585-WJ-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0585-WJ-S(P)) | ELISA | Human scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-027 | Afuco™ Anti-CD40LG ADCC Recombinant Antibody (Ruplizumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-876 | Afuco™ Anti-CD40LG ADCC Recombinant Antibody (Toralizumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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