Human Anti-CD40LG (clone YMF323.6) scFv-Fc Chimera

Name: Human Anti-CD40LG (clone YMF323.6) scFv-Fc Chimera
SKU: VS-0425-FY79
Rating: 5 (1 reviews)

CAT#: VS-0425-FY79

This fusion protein targets CD40LG (CD40 ligand), a co-stimulatory molecule that plays a crucial role in immune cell interactions. It is designed to modulate immune responses and has potential applications in cancer immunotherapy and autoimmune diseases.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line JURKAT

Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2010_G3_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line HEL

Immunofluorescent staining of human cell line HEL shows localization to golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2069_B6_2_blue_green.jpg

Normal Tissue Figure 3 Colon

Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_3.jpg

Normal Tissue Figure 4 Lymph node

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enriched (MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG

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Specifications

Immunogen
The immunogen is recombinant human CD40L expressed on the surface of CHO cells.

Host Species
Human

Type
Human IgG1, scFv-Fc

Antibody Isotype
scFv-Fc

Specificity
Human CD40LG

Species Reactivity
Human

Clone
YMF323.6

Applications
Flow Cytometry, ELISA, Immunohistochemistry

Related Disease
Idiopathic Anterior Uveitis and Cd3zeta Deficiency

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Flow Cytometry: 0.1-10 μg/mL
Immunohistochemistry: 1-10 μg/mL
The optimal dilution concentration is determined by the experiment.

Target

Alternative Names
CD40 Ligand; TNF-Related Activation Protein; Tumor Necrosis Factor (Ligand) Superfamily Member 5; T-B Cell-Activating Molecule; CD40 Antigen Ligand; T-Cell Antigen Gp39; TNFSF5; CD40-L; CD40L; TRAP; Tumor Necrosis Factor (Ligand) Superfamily, Member 5 (Hyper-IgM Syndrome)

Gene ID
959

UniProt ID
P29965

Long Name
CD40 Ligand

Sequence Similarities
Belongs to the tumor necrosis factor family.

Cellular Localization
Cell membrane; Single-pass type II membrane protein; Cell surface

Post Translation Modifications
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type.
Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.
Glycosylation at Asn240
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect CD40L_HUMAN (610)
Glycosylation from GlyGen 2 sites, 32 N-linked glycans (2 sites)

Protein Refseq
NP_000065.1

Function
Cytokine that acts as a ligand to CD40/TNFRSF5.
Costimulates T-cell proliferation and cytokine production.
Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation.
Induces the activation of NF-kappa-B.
Induces the activation of kinases MAPK8 and PAK2 in T-cells.
Induces tyrosine phosphorylation of isoform 3 of CD28.
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4.
Involved in immunoglobulin class switching.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0425-FY79.pdf

MSDS

MSDS - VS-0425-FY79.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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