Human Anti-CD40LG (clone YMF323.6) scFv-Fc Chimera (CAT#: VS-0425-FY79)

• Immunogen
• The immunogen is recombinant human CD40L expressed on the surface of CHO cells.

• Host Species
• Human

• Type
• Human IgG1, scFv-Fc

• Specificity
• Human CD40LG

• Species Reactivity
• Human

• Clone
• YMF323.6

• Applications
• Flow Cytometry, ELISA, Immunohistochemistry

• Related Disease
• Idiopathic Anterior Uveitis and Cd3zeta Deficiency

• Purity
• >95% as determined by SDS-PAGE

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Flow Cytometry: 0.1-10 μg/mL
  Immunohistochemistry: 1-10 μg/mL
  The optimal dilution concentration is determined by the experiment.

• Alternative Names
• CD40 Ligand; TNF-Related Activation Protein; Tumor Necrosis Factor (Ligand) Superfamily Member 5; T-B Cell-Activating Molecule; CD40 Antigen Ligand; T-Cell Antigen Gp39; TNFSF5; CD40-L; CD40L; TRAP; Tumor Necrosis Factor (Ligand) Superfamily, Member 5 (Hyper-IgM Syndrome)

• Gene ID
• 959

• UniProt ID
• P29965

• Long Name
• CD40 Ligand

• Sequence Similarities
• Belongs to the tumor necrosis factor family.

• Cellular Localization
• Cell membrane; Single-pass type II membrane protein; Cell surface

• Post Translation Modifications
• The soluble form derives from the membrane form by proteolytic processing.
  N-linked glycan is a mixture of high mannose and complex type.
  Glycan structure does not influence binding affinity to CD40.
  Not O-glycosylated.
  Glycosylation at Asn240
  Modification sites at PhosphoSitePlus
  Glycosylation from GlyConnect CD40L_HUMAN (610)
  Glycosylation from GlyGen 2 sites, 32 N-linked glycans (2 sites)

• Protein Refseq
• NP_000065.1

• Function
• Cytokine that acts as a ligand to CD40/TNFRSF5.
  Costimulates T-cell proliferation and cytokine production.
  Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation.
  Induces the activation of NF-kappa-B.
  Induces the activation of kinases MAPK8 and PAK2 in T-cells.
  Induces tyrosine phosphorylation of isoform 3 of CD28.
  Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4.
  Involved in immunoglobulin class switching.

• TAB-225LC
• Anti-Human CD40LG Recombinant Antibody (YMF323.6)

• TAB-225LC-S(P)
• Anti-Human CD40LG Recombinant Antibody scFv Fragment (YMF323.6)

• TAB-225LC-F(E)
• Anti-Human CD40LG Recombinant Antibody Fab Fragment (YMF323.6)

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