Human Anti-CD40LG (clone YMF323.6) scFv-Fc Chimera
CAT#: VS-0425-FY79
This fusion protein targets CD40LG (CD40 ligand), a co-stimulatory molecule that plays a crucial role in immune cell interactions. It is designed to modulate immune responses and has potential applications in cancer immunotherapy and autoimmune diseases.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2010_G3_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2069_B6_2_blue_green.jpg
Normal Tissue
Figure 3 Colon
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_3.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG
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Specifications
- Immunogen
- The immunogen is recombinant human CD40L expressed on the surface of CHO cells.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human CD40LG
- Species Reactivity
- Human
- Clone
- YMF323.6
- Applications
- Flow Cytometry, ELISA, Immunohistochemistry
- Related Disease
- Idiopathic Anterior Uveitis and Cd3zeta Deficiency
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Flow Cytometry: 0.1-10 μg/mL
Immunohistochemistry: 1-10 μg/mL
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- CD40 Ligand; TNF-Related Activation Protein; Tumor Necrosis Factor (Ligand) Superfamily Member 5; T-B Cell-Activating Molecule; CD40 Antigen Ligand; T-Cell Antigen Gp39; TNFSF5; CD40-L; CD40L; TRAP; Tumor Necrosis Factor (Ligand) Superfamily, Member 5 (Hyper-IgM Syndrome)
- Gene ID
- 959
- UniProt ID
- P29965
- Long Name
- CD40 Ligand
- Sequence Similarities
- Belongs to the tumor necrosis factor family.
- Cellular Localization
- Cell membrane; Single-pass type II membrane protein; Cell surface
- Post Translation Modifications
- The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type.
Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.
Glycosylation at Asn240
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect CD40L_HUMAN (610)
Glycosylation from GlyGen 2 sites, 32 N-linked glycans (2 sites)
- Protein Refseq
- NP_000065.1
- Function
- Cytokine that acts as a ligand to CD40/TNFRSF5.
Costimulates T-cell proliferation and cytokine production.
Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation.
Induces the activation of NF-kappa-B.
Induces the activation of kinases MAPK8 and PAK2 in T-cells.
Induces tyrosine phosphorylation of isoform 3 of CD28.
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4.
Involved in immunoglobulin class switching.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone YMF323.6"
- CAT
- Product Name
See other products for "CD40LG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1071z | Mouse Anti-CD40LG Recombinant Antibody (clone 18C1) | ELISA, FC, FuncS, ICC, IF | Mouse IgG1 |
| PABW-020 | Human Anti-CD40LG Recombinant Antibody (PABW-020) | Neut | Human IgG1, κ |
| HPAB-0179-YC | Human Anti-CD40LG Recombinant Antibody (HPAB-0179-YC) | ELISA | Human IgG1 |
| HPAB-0744LY | Human Anti-CD40LG Recombinant Antibody (HPAB-0744LY) | Inhib | Human IgG |
| VS3-CJ861 | Mouse Anti-CD40LG Recombinant Antibody (clone M91) | IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-027 | Anti-Human CD40L Recombinant Antibody (TAB-027) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
| TAB-876 | Anti-Human CD40 ligand Recombinant Antibody (Toralizumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
| TAB-211LC | Human Anti-CD40LG Recombinant Antibody (TAB-211LC) | ELISA | Human IgG, κ |
| TAB-210LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-210LC-F(E)) | ELISA | Humanized Fab |
| TAB-211LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-211LC-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G080E | Anti-Cd40lg immunotoxin LL2 (IgG)-PE | Cytotoxicity assay, Function study | |
| AGTO-G081E | Anti-Cd40lg immunotoxin LL2 (Fab)-PE | Cytotoxicity assay, Function study | |
| AGTO-L051P | anti-CD40LG immunotoxin MR1 (scFv)-PAP | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-020 | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (PFBW-020) | Neut | Human Fab |
| HPAB-0179-YC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0179-YC-F(E)) | ELISA | Human Fab |
| FAMAB-0135-YC-F(E) | Hamster Anti-CD40LG Recombinant Antibody (clone mr-1); Fab Fragment | ELISA | Hamster Fab |
| HPAB-0744LY-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0744LY-F(E)) | ELISA | Human Fab |
| HPAB-0585-WJ-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0585-WJ-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-020 | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (PSBW-020) | Neut | Human scFv |
| HPAB-0179-YC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0179-YC-S(P)) | ELISA | Human scFv |
| FAMAB-0135-YC-S(P) | Hamster Anti-CD40LG Recombinant Antibody (clone mr-1); scFv Fragment | ELISA | Hamster scFv |
| HPAB-0744LY-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0744LY-S(P)) | ELISA | Human scfv |
| HPAB-0880LY-S(P) | Mouse Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0880LY-S(P)) | FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-212LC | Mouse Anti-Human CD40LG Recombinant Antibody (ABI793) | Block | |
| TAB-212LC-S(P) | Anti-Human CD40LG Recombinant Antibody scFv Fragment (ABI793) | ELISA | |
| TAB-212LC-F(E) | Anti-Human CD40LG Recombinant Antibody Fab Fragment (ABI793) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-225LC | Anti-Human CD40LG Recombinant Antibody (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
| TAB-225LC-S(P) | Anti-Human CD40LG Recombinant Antibody scFv Fragment (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
| TAB-225LC-F(E) | Anti-Human CD40LG Recombinant Antibody Fab Fragment (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-213LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-213LC-S(P)) | FC, ELISA, RBA | |
| TAB-214LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-214LC-S(P)) | FC, ELISA, RBA | |
| TAB-215LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-215LC-S(P)) | FC, ELISA, RBA | |
| TAB-216LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-216LC-S(P)) | FC, ELISA, RBA | |
| TAB-217LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-217LC-S(P)) | FC, ELISA, RBA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-368CQ | Mouse Anti-CD40LG Recombinant Antibody (clone 24-31) | FC, Block, IF | Mouse IgG1, κ |
| NEUT-375CQ | Hamster Anti-CD40LG Recombinant Antibody (clone MR1) | IHC-Fr, FC, IP, Block | Hamster IgG1, κ |
| NEUT-377CQ | Hamster Anti-CD40LG Recombinant Antibody (clone MAB0820) | Block, FC, IHC-Fr | Hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-369CQ | Mouse Anti-CD40LG Recombinant Antibody (clone CBL300) | FC, IHC, CyTOF, Neut, WB | Mouse IgG2b |
| NEUT-370CQ | Mouse Anti-CD40LG Recombinant Antibody (clone LL2) | Neut, ELISA, FC, FuncS, IF | Mouse IgG1 |
| NEUT-371CQ | Mouse Anti-CD40LG Recombinant Antibody (clone LL48) | Neut, FuncS, IF | Mouse IgG2a |
| NEUT-372CQ | Mouse Anti-CD40LG Recombinant Antibody (NEUT-372CQ) | Neut | Mouse IgG2b |
| NEUT-374CQ | Rat Anti-CD40LG Recombinant Antibody (clone CBL719) | FC, CyTOF, Neut | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0561 | Hi-Affi™ Rabbit Anti-CD40LG Recombinant Antibody (clone DS561AB) | WB, FC | Rabbit IgG |
| MOR-0562 | Hi-Affi™ Rabbit Anti-CD40LG Recombinant Antibody (clone DS562AB) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0610LC | Mouse Anti-CD40LG Recombinant Antibody (clone h5c8) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-022CN | Human Anti-CD40LG Recombinant Antibody (NS-022CN) | ELISA, FC | Human IgG |
| NS-022CN-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (NS-022CN-F(E)) | ELISA, FC | Human Fab |
| NS-022CN-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (NS-022CN-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-027 | Afuco™ Anti-CD40LG ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-027) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-876 | Afuco™ Anti-CD40LG ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-876) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC164 | CytoStream™ Rabbit Anti-CD40LG Recombinant Antibody (VS13-YC164) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC389 | Recombinant Anti-CD40LG Vesicular Antibody, EV Displayed (VS-0425-YC389) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1182 | Anti-CD40LG Immunohistochemistry Kit | IHC | |
| VS-0525-XY1183 | Anti-Mouse CD40LG Immunohistochemistry Kit | IHC |
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