Recombinant Rabbit Anti-CDK4 Antibody (clone R04-8I5)
CAT#: VS3-FY347
This product is a recombinant rabbit antibody that recognizes human and mouse CDK4 protein. It can detect a band of approximately 34 kDa (observedd molecular weight: 34 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli, nuclear membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6024/1704_B1_1_cr5811e29350250_selected.jpg
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WB
Figure 1. Recombinant Rabbit Anti-CDK4 Antibody (clone R04-8I5) in WB.
Western blot analysis of Cdk4 in HeLa lysate K562 using CDK4 antibody.
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Specifications
- Immunogen
- A synthetic peptide of human Cdk4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse CDK4
- Species Reactivity
- Human, Mouse
- Clone
- R04-8I5
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 34 kDa; Observed MW: 34 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cyclin Dependent Kinase 4; Cell Division Protein Kinase 4; EC 2.7.11.22; PSK-J3; Cyclin-Dependent Kinase 4; EC 2.7.11; CMM3
- Gene ID
- 1019
- UniProt ID
- P11802
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
- Cellular Localization
- Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
- Protein Refseq
- NP_000066.1
- Function
- Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CDK4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0113MZ | Chicken Anti-CDK4 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0630 | Hi-Affi™ Rabbit Anti-CDK4 Recombinant Antibody (clone DS630AB) | ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0339-CN | Mouse Anti-CDK4 Recombinant Antibody (clone 5-C3-G3) | WB, IF, IHC | Mouse IgG2c |
| MRO-0340-CN | Rabbit Anti-CDK4 Recombinant Antibody (clone CBACN-138) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-0341-CN | Rabbit Anti-CDK4 Recombinant Antibody (clone CBACN-139) | WB, IF, IP | Rabbit IgG |
| VS3-FY346 | Recombinant Rabbit Anti-CDK4 Antibody (clone R04-4I5) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-FY348 | Recombinant Rabbit Anti-CDK4 Antibody (clone R07-4G7) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC569 | AbPlus™ Anti-CDK4 Magnetic Beads (VS-0724-YC569) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY404 | Anti-CDK4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1317 | Anti-Mouse CDK4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1316 | Anti-Human CDK4 Immunohistochemistry Kit | IHC |
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