Recombinant Mouse Anti-EGF Capture Antibody (clone 6B8) (CAT#: VS3-WK1664)
This antibody is a mouse recombinant antibody against human EGF. The sandwich ELISA was established using matched antibody pair 6B8 and 5B3. The clone 6B8 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It can also used in WB analysis at a dilution of 1/500-1000. It was developed by immunization of mouse with recombinant human EGF protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG1.
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RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (EFO-21, SK-BR-3, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138798-EGF
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138798-EGF
Specifications
- Immunogen
- Recombinant human EGF protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human EGF
- Species Reactivity
- Human
- Clone
- 6B8
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
ELISA: 1/1000
Target
- Alternative Names
- Epidermal Growth Factor; Epidermal Growth Factor (Beta-Urogastrone); Pro-Epidermal Growth Factor; Beta-Urogastrone; HOMG4; URG
- Gene ID
- 1950
- UniProt ID
- P01133
- Long Name
- Epidermal Growth Factor
- Cellular Localization
- Membrane
- Post Translation Modifications
- O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
- Protein Refseq
- NP_001171601.1
- Function
- EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-305-F(E) | Recombinant Anti-human EGF Antibody Fab Fragment | IF, FuncS | Fab |
| MHH-305-F(E) | Recombinant Human Anti-human EGF Antibody Fab Fragment | RIA, WB, FuncS | Fab |
| HPAB-0157-WJ-F(E) | Mouse Anti-EGF Recombinant Antibody (clone 0914); Fab Fragment | ELISA, FC | Mouse Fab |
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