Recombinant Mouse Anti-EGF Detection Antibody (clone 5B3)
CAT#: VS3-WK1665
This antibody is a mouse recombinant antibody against human EGF. The sandwich ELISA was established using matched antibody pair 6B8 and 5B3. The clone 5B3 can be used in ELISA analysis as a detection antibody at a dilution of 1/1000. It can also used in WB analysis at a dilution of 1/500-1000. It was developed by immunization of mouse with recombinant human EGF protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2a.
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (EFO-21, SK-BR-3, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138798-EGF
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Specifications
- Immunogen
- Recombinant human EGF protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human EGF
- Species Reactivity
- Human
- Clone
- 5B3
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
ELISA: 1/1000
Target
- Alternative Names
- Epidermal Growth Factor; Epidermal Growth Factor (Beta-Urogastrone); Pro-Epidermal Growth Factor; Beta-Urogastrone; HOMG4; URG
- Gene ID
- 1950
- UniProt ID
- P01133
- Long Name
- Epidermal Growth Factor
- Cellular Localization
- Membrane
- Post Translation Modifications
- O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
- Protein Refseq
- NP_001171601.1
- Function
- EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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