Recombinant Mouse Anti-FGF2 Detection Antibody (clone 3D1) (CAT#: VS3-WK1633)
This antibody is a mouse recombinant antibody against human FGF2. The sandwich ELISA was established using matched antibody pair 4G11 and 3D1. The clone 3D1 can be used in ELISA analysis as a detection antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human FGF2 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG1.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/641_G7_4_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/641_G7_4_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/640_G7_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/640_G7_1_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/642_G7_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/642_G7_1_red_green.jpg
Figure 2 IHC staining of human caudate
(Immunohistochemical staining of human caudate shows nuclear positivity in glia.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/65502/1336_684_200x200.jpg
(Immunohistochemical staining of human caudate shows nuclear positivity in glia.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/65502/1336_684_200x200.jpg
Figure 5 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_9_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_9_5.jpg
Figure 6 Caudate
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_8_7.jpg
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_8_7.jpg
Figure 7 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_8_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_8_3.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_9_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_9_5.jpg
Figure 9 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-HME1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138685-FGF2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138685-FGF2
Specifications
- Immunogen
- Recombinant human FGF2 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human FGF2
- Species Reactivity
- Human
- Clone
- 3D1
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.01% thimerosal
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Fibroblast Growth Factor 2; Fibroblast Growth Factor 2 (Basic); Heparin-Binding Growth Factor 2; HBGF-2; FGF-2; BFGF
- Gene ID
- 2247
- UniProt ID
- P09038
- Long Name
- Fibroblast Growth Factor 2
- Sequence Similarities
- Belongs to the heparin-binding growth factors family.
- Cellular Localization
- Nucleus, Secreted
- Post Translation Modifications
- Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
- Protein Refseq
- NP_001997.5
- Function
- Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4.
Also acts as an integrin ligand which is required for FGF2 signaling.
Binds to integrin ITGAV:ITGB3.
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-357-F(E) | Recombinant Anti-human FGF2 Antibody Fab Fragment | IF, FC, FuncS | Fab |
| MHH-357-F(E) | Recombinant Human Anti-human FGF2 Antibody Fab Fragment | IP, IF, Biosensors, FuncS | Fab |
| HPAB-0164-WJ-F(E) | Mouse Anti-FGF2 Recombinant Antibody (clone E12); Fab Fragment | ELISA | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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