Recombinant Rabbit Anti-FLT1 Detection Antibody (clone R74-3T-9) (CAT#: VS3-WK1947)
This antibody is a rabbit recombinant antibody against human FLT1. It was developed by immunization of rabbit with recombinant human FLT1 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R83-8I-2 and R74-3T-9 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R74-3T-9 can be used in ELISA analysis to identify human FLT1.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/90_G3_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/90_G3_2_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/1368_A5_2_blue_green.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/1368_A5_2_blue_green.jpg
Figure 3 Testis
(Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/142518_A_6_6.jpg
(Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/142518_A_6_6.jpg
Figure 4 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, HMC-1, HUVEC TERT2, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102755-FLT1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102755-FLT1
Figure 4 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, HMC-1, HUVEC TERT2, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102755-FLT1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102755-FLT1
Specifications
- Immunogen
- Recombinant human FLT1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FLT1
- Species Reactivity
- Human
- Clone
- R74-3T-9
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Fms Related Tyrosine Kinase 1; Vascular Permeability Factor Receptor; Fms-Related Tyrosine Kinase 1 (Vascular Endothelial Growth Factor/Vascular Permeability Factor Receptor); Vascular Endothelial Growth Factor Receptor 1; Tyrosine-Protein Kinase Receptor FLT; Tyrosine-Protein Kinase FRT; Fms-Like Tyrosine Kinase 1; EC 2.7.10.1
- Gene ID
- 2321
- UniProt ID
- P17948
- Long Name
- Fms Related Tyrosine Kinase 1
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Endosome, Membrane, Secreted
- Post Translation Modifications
- N-glycosylated.
Ubiquitinated after VEGFA-mediated autophosphorylation, leading to proteolytic degradation.
- Protein Refseq
- NP_001153392.1
- Function
- Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1152 | Human Anti-FLT1 Recombinant Antibody (clone 867.2A3); scFv Fragment | ELISA, WB, IHC, IP | Human scFv |
| DrMAB-1158 | Human Anti-FLT1 Recombinant Antibody (clone 867.2D2); scFv Fragment, HRP | ELISA, WB, IHC, IP | Human scFv |
| HPAB-0137-YC-S(P) | Human Anti-FLT1 Recombinant Antibody (clone 18F1); scFv Fragment | ELISA, FC, Block, WB | Human scFv |
| HPAB-0785-CN-S(P) | Mouse Anti-FLT1 Recombinant Antibody (clone 1-833-527); scFv Fragment | ELISA, FC | Mouse scFv |
| HPAB-M0695-YC-S(P) | Mouse Anti-FLT1 Recombinant Antibody; scFv Fragment (HPAB-M0695-YC-S(P)) | ELISA, WB, FuncS | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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