Recombinant Rabbit Anti-Mouse MMP9 Capture Antibody (clone R35-9W-2)

Name: Recombinant Rabbit Anti-Mouse MMP9 Capture Antibody (clone R35-9W-2)
SKU: VS3-WK1874
Rating: 5 (1 reviews)

CAT#: VS3-WK1874

This antibody is a rabbit recombinant antibody against mouse MMP9. It was developed by immunization of rabbit with recombinant mouse MMP9 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R35-9W-2 and R61-1P-4. The clone R35-9W-2 can be used as a capture antibody in ELISA analysis to identify mouse MMP9.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg

Subcellular Location Figure 2 IHC staining of human bone marrow

Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg

Subcellular Location Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg

Normal Tissue Figure 5 Testis

Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg

Normal Tissue Figure 6 Lymph node

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enriched (NB-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9

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Specifications

Immunogen
Recombinant mouse MMP9 protein

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Mouse MMP9

Species Reactivity
Mouse

Clone
R35-9W-2

Applications
ELISA

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Protein A affinity purified

Purity
>95% as determined by SDS-PAGE

Aggregation
< 5%

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
0.01M PBS, pH 7.2

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Target

Alternative Names
Clg4b; Gel B; MMP-9; B/MMP9; pro-MMP-9

Gene ID
17395

UniProt ID
P41245

Long Name
Matrix metallopeptidase 9

Sequence Similarities
Belongs to the peptidase M10A family.

Cellular Localization
Extracellular matrix, Secreted

Post Translation Modifications
N- and O-glycosylated.

Protein Refseq
NP_038627.1

Function
Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (By similarity).
Could play a role in bone osteoclastic resorption.

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Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-WK1874.pdf

MSDS

MSDS - VS3-WK1874.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Detection antibody

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Secondary Antibody

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