Recombinant Rabbit Anti-MMP9 Detection Antibody (clone R07-8E3)
CAT#: VS3-WK1741
This antibody is a rabbit recombinant antibody against human MMP9. It was developed by immunization of rabbit with recombinant human MMP9 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R04-8Z7 and R07-8E3 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R07-8E3 can be used in ELISA analysis to identify human MMP9.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Testis
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
❮
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Specifications
- Immunogen
- Recombinant human MMP9 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MMP9
- Species Reactivity
- Human
- Clone
- R07-8E3
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Matrix Metallopeptidase 9; Matrix Metalloproteinase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase); EC 3.4.24.35; CLG4B; MMP-9; GELB; Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase); Matrix Metalloproteinase-9
- Gene ID
- 4318
- UniProt ID
- P14780
- Long Name
- Matrix Metallopeptidase 9
- Sequence Similarities
- Belongs to the peptidase M10A family.
- Cellular Localization
- Extracellular matrix, Secreted
- Protein Refseq
- NP_004985.2
- Function
- Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption (By similarity).
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1048 | Recombinant Mouse Anti-Human MMP9 Antibody Fab Fragment | LMNX, ELISA, WB, IF | Fab |
| PFBC-174 | Mouse Anti-MMP9 Recombinant Antibody Fab Fragment (PFBC-174) | ELISA, Neut | Mouse Fab |
| HPAB-0199-FY-F(E) | Human Anti-MMP9 Recombinant Antibody (clone DX-2802); Fab Fragment | ELISA, FC | Human Fab, λ |
| HPAB-0200-FY-F(E) | Human Anti-MMP9 Recombinant Antibody (clone M0279-A03); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-M0359-YC-F(E) | Human Anti-MMP9 Recombinant Antibody (clone AB0045); Fab Fragment | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-533CL | Anti-Human MMP9 Recombinant Antibody (TAB-533CL) | WB, Inhib | Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0824CL | Human Anti-MMP9 Recombinant Antibody (TAB-0824CL) | ELISA | Human IgG4 |
| TAB-0825CL | Human Anti-MMP9 Recombinant Antibody (TAB-0825CL) | ELISA | Human IgG4 |
| TAB-0824CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0824CL-S(P)) | ELISA | Human scFv |
| TAB-0824CL-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0824CL-F(E)) | ELISA | Human Fab |
| TAB-0825CL-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0825CL-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0826CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0826CL) | Inhib, ELISA, IHC | Mouse IgG |
| TAB-0827CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0827CL) | ELISA | Mouse IgG |
| TAB-0826CL-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0826CL-S(P)) | ELISA | Mouse scFv |
| TAB-0826CL-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0826CL-F(E)) | ELISA | Mouse Fab |
| TAB-0827CL-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0827CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-174 | Mouse Anti-MMP9 Recombinant Antibody scFv Fragment (PSBC-174) | ELISA, Neut | Mouse scFv |
| HPAB-0027-YC-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (HPAB-0027-YC-S(P)) | FC | Mouse scFv |
| HPAB-0028-YC-S(P) | Human Anti-MMP9 Recombinant Antibody (clone G03-VH-GL-V1/ G06-VL-GL1); scFv Fragment | Neut | Human scFv |
| HPAB-M0311-YC-S(P) | Mouse Anti-MMP9 Recombinant Antibody (clone AB0046); scFv Fragment | ELISA | Mouse scFv |
| HPAB-M0359-YC-S(P) | Human Anti-MMP9 Recombinant Antibody (clone AB0045); scFv Fragment | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0717MZ | Chicken Anti-MMP9 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1748CQ | Recombinant Mouse Anti-MMP9 Antibody (VMC204) | Inhib, IHC-Fr, IP, IHC-P, WB, IHC, ICC | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2263 | Hi-Affi™ Recombinant Rabbit Anti-MMP9 Monoclonal Antibody (DS2263AB) | FC, IHC-P, WB | IgG |
| MOR-4331 | Hi-Affi™ Recombinant Rabbit Anti-MMP-9 Monoclonal Antibody (SI420DS) | ELISA | IgG |
| MOR-4332 | Hi-Affi™ Recombinant Rabbit Anti-MMP-9 Monoclonal Antibody (SI421DS) | ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-002ML | Anti-Human MMP9 Recombinant Antibody (TAB-002ML) | ELISA, IHC, FC, IP, IF, FuncS | IgG4, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0027-YC | Mouse Anti-MMP9 Recombinant Antibody (HPAB-0027-YC) | FC | Mouse IgG2b, κ |
| HPAB-0028-YC | Human Anti-MMP9 Recombinant Antibody (clone G03-VH-GL-V1/ G06-VL-GL1) | Neut | Human IgG4 |
| ZG-0471U | Rabbit Anti-MMP9 Recombinant Antibody (clone 29C11) | ELISA, IHC, FC | Rabbit IgG |
| VS3-XY1091 | Mouse Anti-MMP9 Recombinant Antibody (clone 5G3) | ELISA, WB, IHC, FC, ICC | Mouse IgG2a |
| VS3-XY1092 | Mouse Anti-MMP9 Recombinant Antibody (clone 2G3) | WB, IP, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-002ML | Afuco™ Anti-MMP9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-002ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX783 | Mouse Anti-MMP9 Recombinant Antibody (clone 331CT17.4.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY101 | Human Anti-MMP9 (clone DX-2802) scFv-Fc Chimera | WB, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1357 | Anti-MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4460 | Anti-Mouse MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4461 | Anti-Zebrafish MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4459 | Anti-Human MMP9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC735 | CytoStream™ Rabbit Anti-MMP9 Recombinant Antibody (VS13-YC735) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC228 | Recombinant Anti-MMP9 Eliminating Antibody, pH-Sensitive (VS-0625-YC228) | Antigen-Sweeping In Vivo. | Human IgG4 kappa |
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