10-FnIII scaffold protein anti-Human VEGFR2 (CAT#: SFN-L030)
The tenth fibronectin type III domain (10Fn3) that bound VEGFR2 was isolated from the library using mRNA Display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=0.59 nM) for VEGFR2 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to nuclear membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73577/1825_D8_31_cr5ac340707076b_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to nuclear membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73577/1825_D8_31_cr5ac340707076b_selected.jpg
Figure 2 Placenta
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_2_7_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_2_7_val_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_8_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_8_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_7_5.jpg
Figure 5 Testis
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_6_6.jpg
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_6_6.jpg
Figure 6 RNA cell line category: Group enriched (HUVEC TERT2, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128052-KDR
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128052-KDR
Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2–3 loops
- Target
- VEGFR2
- Species Reactivity
- Human
- Expression Host
- E. coli
- Affinity Constant
- 0.59 nM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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