Recombinant Anti-human AKT2 VHH Single Domain Antibody
CAT#: NAB-49-sdAb
Anti-human AKT2 VHH Single Domain Antibody is a recombinant protein produced in E. coli.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1304_E6_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity(granular pattern) in purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1518_H7_2_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1323_E6_2_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105221-AKT2
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Specifications
- Immunogen
- human v-akt murine thymoma viral oncogene homolog 2
- Host Species
- Llama
- Type
- Llama VHH
- Species Reactivity
- Human
- Molecule Class
- VHH Single Domain Antibody
- Applications
- This product is suitable for use in IP, FC, ChiP, Neut and most other immunological methods.
Product Property
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Related Signaling Pathways
BCR Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Ras Signaling Pathway
Related Diseases
Breast Cancer
Colorectal Cancer
Gastric Cancer
Hepatocellular Carcinoma
Non-small Cell Lung Cancer
Prostate Cancer
Endocrine Resistance
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "AKT2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0033MZ | Chicken Anti-AKT2 Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0128 | Hi-Affi™ Rabbit Anti-AKT2 Recombinant Antibody (clone DS128AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX35 | Mouse Anti-AKT2 Recombinant Antibody (clone 148CT5.4.1.4) | WB, ICC, IHC | Mouse IgG1 |
| VS3-QX36 | Mouse Anti-AKT2 Recombinant Antibody (clone 7F12-H0-B7) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-CJ874 | Mouse Anti-AKT2 Recombinant Antibody (VS3-CJ874) | WB, ICC, IF, IHC, IP, ChIP | Mouse IgG |
| VS3-XY37 | Mouse Anti-AKT2 Recombinant Antibody (clone 2A1) | WB, IP, IF, IHC | Mouse IgG1 |
| VS7-HM69 | Mouse Anti-AKT2 Recombinant Antibody (clone CBL025HM) | WB, IHC-p, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY16 | Mouse Anti-NHP AKT2 Recombinant Antibody (clone 1B6) | WB, IHC, ICC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY284 | Anti-AKT2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY285 | Anti-Mouse AKT2 Immunohistochemistry Kit | IHC |
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