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    Mouse Anti-NHP AKT2 Recombinant Antibody (clone 1B6) (CAT#: VS-1024-XY16)

    This mouse-derived monoclonal antibody targets AKT2 with proven reactivity to human, non-human primates, and rat tissues. Generated using a purified recombinant fragment of human AKT2 as the immunogen, it provides exceptional specificity and reliability. Suitable for a range of applications including Western blot (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC), this antibody is a versatile tool for investigating AKT2 expression and function across various biological contexts. Its broad reactivity and robust performance make it a valuable asset in cellular and molecular research.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A549
    Figure 1 IF staining of human cell line A549
    (Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, cytosol & vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1304_E6_3_selected.jpg
    Figure 2 IHC staining of human cerebellum
    Figure 2 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity(granular pattern) in purkinje cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/ihc_selected.jpg
    Figure 3 IF staining of human cell line RT4
    Figure 3 IF staining of human cell line RT4
    (Immunofluorescent staining of human cell line RT4 shows localization to cytosol & vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1518_H7_2_blue_red_green.jpg
    Figure 4 IF staining of human cell line U-2 OS
    Figure 4 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1323_E6_2_blue_red_green.jpg
    Normal Tissue
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Endothelial cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_B_9_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_7_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Cells in seminiferous ducts
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_4_6.jpg
    Figure 10 Lymph node
    Figure 10 Lymph node
    (Germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_8.jpg
    RNA Expression
    Figure 11 RNA cell line category: Low cell line specificity
    Figure 11 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105221-AKT2

    Specifications

    • Immunogen
    • Purified recombinant fragment of human AKT2
    • Host Species
    • Mouse
    • Type
    • Mouse IgG2b
    • Specificity
    • AKT2
    • Species Reactivity
    • Human, Non-human primate, Rat
    • Clone
    • 1B6
    • Applications
    • WB, IHC, ICC

    Product Property

    • Purification
    • Protein A purified
    • Purity
    • >95%, determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • PBS, pH 7.4
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

    Applications

    • Application Notes
    • WB: 1:500-1:1000, IHC: 1:200-1:800, ICC: 1:200-1:800

    Target

    • Alternative Names
    • HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA

    Recommended Products

    Recommended Dilution Buffer

    Isotype Control

    Secondary Antibody

    Related Resources

    • Related Signaling Pathways
    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    CAT Product Name Application Type
    NAB-49-sdAb Recombinant Anti-human AKT2 VHH Single Domain Antibody IP, FC, ChiP, Neut Llama VHH

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