Rabbit Anti-AKT2 Recombinant Antibody (clone D16-H)
CAT#: ZG-0383U
Rabbit anti-AKT2 antibody (clone D16-H) displayed high specificity against the AKT2 protein of Human, mouse and rat.
Tested Data
Gene Expression
IF
Figure 1 Rabbit Anti-AKT2 Antibody (ZG-0383U) in IF
Representative pictures of Akt2 expression in Akt2 transfected HEK293 cells, visualized with anti-Akt2 rabbit monoclonal antibody. Primary antibody dilution 1:300.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1304_E6_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity(granular pattern) in purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1518_H7_2_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1323_E6_2_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105221-AKT2
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Specifications
- Immunogen
- Peptide derived from the C-terminal sequence of human AKT2 protein. Antibody recognizes the epitope located between Arg455 - Thr468.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AKT2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- D16-H
- Applications
- ELISA, WB, IP, ICC
- Conjugate
- Unconjugated
- Related Disease
- Hypoinsulinemic Hypoglycemia With Hemihypertrophy and Type 2 Diabetes Mellitus.
Product Property
- Purification
- Affinity Purified
- Buffer
- 20 mM Tris-HCl, pH 8.0, 10 mg/ml BSA and 0.05% sodium azide
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation, Immunocytochemistry.
Target
- Alternative Names
- PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; RAC-beta serine/threonine-protein kinase; PKB beta; RAC-PK-beta; murine thymoma viral (v-akt) homolog-2; protein kinase Akt-2; protein kinase B beta; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase beta; v-akt murine thymoma viral oncogene homolog 2
- Gene ID
- 208
- UniProt ID
- P31751
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Related Signaling Pathways
BCR Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Ras Signaling Pathway
Related Diseases
Breast Cancer
Colorectal Cancer
Gastric Cancer
Hepatocellular Carcinoma
Non-small Cell Lung Cancer
Prostate Cancer
Endocrine Resistance
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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