Hi-Affi™ Rabbit Anti-AKT2 Recombinant Antibody (clone DS128AB)
CAT#: MOR-0128
Recombinant Rabbit anti-AKT2 monoclonal antibody for WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1304_E6_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity(granular pattern) in purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1518_H7_2_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1323_E6_2_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105221-AKT2
❮
❯
Specifications
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Human
- Clone
- DS128AB
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Related Signaling Pathways
BCR Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Ras Signaling Pathway
Related Diseases
Breast Cancer
Colorectal Cancer
Gastric Cancer
Hepatocellular Carcinoma
Non-small Cell Lung Cancer
Prostate Cancer
Endocrine Resistance
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| ZG-0274F | Mouse Anti-AKT2 Recombinant Antibody (ZG-0274F) | WB, IHC, IF, ELISA | Mouse IgG |
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| CAT | Product Name | Application | Type |
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| VS-1024-XY16 | Mouse Anti-NHP AKT2 Recombinant Antibody (clone 1B6) | WB, IHC, ICC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
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| VS-0525-XY284 | Anti-AKT2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY285 | Anti-Mouse AKT2 Immunohistochemistry Kit | IHC |
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