Recombinant Anti-human BSG Intrabody [+36 GFP]

Name: Recombinant Anti-human BSG Intrabody [+36 GFP]
SKU: IAB-B050(G)
Rating: 5 (1 reviews)

CAT#: IAB-B050(G)

Recombinant anti-BSG intrabody in fusion with +36GFP works within cells and is directed against a human leukocyte surface molecule BSG, expressed in CHO cells. The intrabody has the ability to down-regulate EMMPRIN cell surface expression on colorectal cancer cell.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HEK 293

Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74870/1466_A2_3_selected.jpg

Normal Tissue Figure 2 Testis

Sertoli cells Staining: High Intensity: Strong Quantity:>75%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_A_4_6.jpg

Normal Tissue Figure 3 Heart muscle

Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_B_6_6.jpg

Normal Tissue Figure 4 Cerebral cortex

Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_B_7_5.jpg

Normal Tissue Figure 5 Colon

Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_A_7_3.jpg

Normal Tissue Figure 6 Kidney

Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_A_7_5.jpg

RNA Expression Figure 7 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172270-BSG

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Specifications

Immunogen
human basigin (Ok blood group)

Host Species
human

Type
scFv-(+36GFP)

Cell Penetrating Peptide
+36 GFP
GFP with a theoretical net charge of +36 (+36 GFP) penetrated several different mammalian cell lines at very low (nanomolar) concentrations. Cationic peptides and proteins have been observed to penetrate cells, and both natural and synthetic cell-penetrating peptides are widely used as delivery vehicles. However, whereas a covalent link between the cell-penetrating peptide and its cargo is typically required.

Species Reactivity
human

Applications
Used for immunoassay techniques such as: Immunofluorescence; Flow Cytometry; Functional studies

Product Property

Purity
>95%, by SDS-PAGE with Coomassie Brilliant Blue staining

Storage
4°C or -20°C, avoid repeated freezing and thawing.

Target

Alternative Names
BSG; basigin (Ok blood group); M6; OK; 5F7; TCSF; CD147; EMMPRIN; basigin; CD147 antigen; OK blood group antigen; collagenase stimulatory factor; leukocyte activation antigen M6; extracellular matrix metalloproteinase inducer; tumor cell-derived collagena

Gene ID
682

UniProt ID
P35613

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - IAB-B050(G).pdf

MSDS

MSDS - IAB-B050(G).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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