Human Anti-CA9 Recombinant Antibody (HPAB-0360-CN) (CAT#: HPAB-0360-CN)
This product is a recombinant Human antibody that recognizes CA9. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Anti-CA9 Recombinant Antibody (HPAB-0360-CN) in WB
Western blot analysis of HPAB-0360-CN was performed by loading Human CA9 Protein (His Tag) in reduced condition (Lane 1) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0360-CN (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 2 Anti-CA9 Recombinant Antibody (HPAB-0360-CN) in DB
Dot Blot analysis of HPAB-0360-CN was performed by coating with Human CA9 Protein (His Tag).
HPAB-0360-CN incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
ELISA
Figure 3 Anti-CA9 Recombinant Antibody (HPAB-0360-CN) in ELISA
ELISA analysis of HPAB-0360-CN was performed by coating with Human CA9 Protein (His Tag). Then blocked with BSA and incubated with HPAB-0360-CN. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Chimeric (Mouse/Human)
- Type
- Chimeric (Mouse/Human) IgG1, κ
- Specificity
- Human CA9
- Species Reactivity
- Human, Cynomolgus
- Applications
- WB, ELISA, FC
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Carbonic Anhydrase IX; Renal Cell Carcinoma-Associated Antigen G250; RCC-Associated Protein G250; RCC-Associated Antigen G250; Carbonate Dehydratase IX; Carbonic Dehydratase; Membrane Antigen MN; P54/58N; CA-IX; CAIX; PMW1; MN; EC 4.2.1.1; G250
- Gene ID
- 768
- UniProt ID
- Q16790
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1276z | Mouse Anti-CA9 Recombinant Antibody (clone 21C7) | ELISA, FC, IHC, WB | Mouse IgG1, κ |
| MRO-0229-CN | Rabbit Anti-CA9 Recombinant Antibody (clone CBACN-083) | WB, IHC | Rabbit IgG |
| HPAB-1174WJ | Mouse Anti-CA9 Recombinant Antibody (clone AB-MN-21.5.2) | ADCC | Mouse IgG1 |
| HPAB-1175WJ | Mouse Anti-CA9 Recombinant Antibody (clone AB-MN-21.6.1) | ADCC | Mouse IgG1 |
| ZG-0578J | Rabbit Anti-CA9 Recombinant Antibody (clone 4F12) | ELISA, IHC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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