Anti-Human CD2 Recombinant Antibody (BTI-322 (also known as Lo-CD2a)) (CAT#: TAB-1631CL)

This antibody is a mouse derived monoclonal antibody that targets to CD2 receptor. It can be used for prevention of first-time kidney rejection, and resistant rejection.


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FuncS

Figure 1 Effect of BTI-322 on primary and secondary xenogeneic MLR.

Figure 1 Effect of BTI-322 on primary and secondary xenogeneic MLR.

(a) Human PBMCs (1 × 10⁶ /ml) were cultured at a 1 : 1 ratio with irradiated SLAᵈᵈ porcine PBMCs, in the presence of 200 ng/ml BTI-322 or the humanized version MEDI-507, or control isotype-matched Ig, followed by measurement of [³H]-TdR incorporation (cpm) at days 3, 5, and 7. (b) Cells harvested after primary xenogeneic stimulation in the presence of these antibodies or controls were cultured for 3 days without stimulant, and then subjected to a secondary xenogeneic MLR, followed by measurement of [³H]-TdR incorporation (cpm) at days 3 and 5.

Xu, Y., Kolber‐Simonds, D., Hope, J. A., Bazin, H., Latinne, D., Monroy, R.,... & SCHUURMAN, H. J. (2004). The anti‐CD2 monoclonal antibody BTI‐322 generates unresponsiveness by activation‐associated T cell depletion. Clinical & Experimental Immunology, 138(3), 476-483.

Figure 2 Use of T cells as responder cells and effect of monocytes.

Figure 2 Use of T cells as responder cells and effect of monocytes.

(a)PBMCs or nylon-wool-purified T cells (both 1 × 10⁶ /ml) were cultured at a 1 : 1 ratio with irradiated SLAᵈᵈ porcine PBMCs, in the presence of 200 ng/ml BTI-322 or control Ig, followed by measurement of [³H]-TdR incorporation (cpm) at days 3, 5, and 7. (b) Purified responder T cells were supplemented with monocytes (adherent cells) at relative proportions [100% reflects the original proportion in the original PBMC preparation (10-20%)].

Xu, Y., Kolber‐Simonds, D., Hope, J. A., Bazin, H., Latinne, D., Monroy, R.,... & SCHUURMAN, H. J. (2004). The anti‐CD2 monoclonal antibody BTI‐322 generates unresponsiveness by activation‐associated T cell depletion. Clinical & Experimental Immunology, 138(3), 476-483.

FuncS

Figure 3 Effect of BTI-322 on secondary MLR: SLAᶜᶜ, third-party SLAᵈᵈ and allogeneic restimulation after primary stimulation with SLAᵈᵈ cells.

Figure 3 Effect of BTI-322 on secondary MLR: SLAᶜᶜ, third-party SLAᵈᵈ and allogeneic restimulation after primary stimulation with SLAᵈᵈ cells.

(a) Cells (1 × 10⁶/ml) from primary 7-day xenogeneic MLR (1 : 1 responder : stimulator ratio) in the presence of 200 ng/ml BTI-322 or control Ig were cultured in secondary MLR with cells of the original SLA haplotype, third-party xenogeneic cells and allogeneic cells. Data shown are [³H]-TdR incorporation at various time-points during secondary culture,or cells cultured during the primary MLR. (b) The same experiment, using allogeneic stimulation in primary MLR. (c) The same experiment using suboptimal xenogeneic stimulation in primary MLR (1 : 0.125 responder : stimulator ratio).

Xu, Y., Kolber‐Simonds, D., Hope, J. A., Bazin, H., Latinne, D., Monroy, R.,... & SCHUURMAN, H. J. (2004). The anti‐CD2 monoclonal antibody BTI‐322 generates unresponsiveness by activation‐associated T cell depletion. Clinical & Experimental Immunology, 138(3), 476-483.

FuncS

Figure 4 Effect of BTI-322 on primary and secondary stimulation with anti TCR Vβ family-specific antibodies.

Figure 4 Effect of BTI-322 on primary and secondary stimulation with anti TCR Vβ family-specific antibodies.

(a) PBMCs were incubated in primary culture with 10 μg/ml anti-TCR Vβ8 antibody, with or without BTI-322 or control Ig (100 ng/ml): [³H]-TdR incorporation was measured at day 7. (b) Cells after primary stimulation as in (a) were washed and cultured for 3 days without stimulator, and then subjected to secondary stimulation with the original anti-Vβ8 antibody or an irrelevant anti-Vβ13 antibody (10 μg/ml): [³H]-TdR incorporation was measured at day 3.

Xu, Y., Kolber‐Simonds, D., Hope, J. A., Bazin, H., Latinne, D., Monroy, R.,... & SCHUURMAN, H. J. (2004). The anti‐CD2 monoclonal antibody BTI‐322 generates unresponsiveness by activation‐associated T cell depletion. Clinical & Experimental Immunology, 138(3), 476-483.

FuncS

Figure 5 Presence of CD3⁺Vβ8⁺ cells after stimulation of PBMCs with anti-TCR Vβ8 or anti-TCR Vβ13 antibody in the presence of BTI-
322 or control Ig.

Figure 5 Presence of CD3⁺Vβ8⁺ cells after stimulation of PBMCs with anti-TCR Vβ8 or anti-TCR Vβ13 antibody in the presence of BTI- 322 or control Ig.

PBMCs (1 × 10⁶/ml) were stimulated for 7 days by control antibody (a,b), anti-Vβ8 antibody (c,d) or anti-Vβ13 antibody (e,f) at 100 ng/ml, in the presence of 100 ng/ml control antibody (a,c,e) or BTI-322 (b,d,f). Subsequently, flow cytometry was performed with a fluorescein isothiocyanate (FITC)-conjugated anti-CD3 antibody in combination with an anti-TCR Vβ8 antibody [indirect staining using a phycoerythrin (PE)-conjugated secondary antibody]. The position of the CD3⁺Vβ8⁺ double-positive population in the individual plots is indicated by circles, determined by flow cytometry on freshly isolated PBMCs from the same donor with appropriate isotypematched control antibody and single-antibody staining. The CD3⁺Vβ8⁺ double-positive population comprises approximately 4.5% of the viable cells in (a,b,e and f); approximately 14% in (c); and approximately 1% in (d).

Xu, Y., Kolber‐Simonds, D., Hope, J. A., Bazin, H., Latinne, D., Monroy, R.,... & SCHUURMAN, H. J. (2004). The anti‐CD2 monoclonal antibody BTI‐322 generates unresponsiveness by activation‐associated T cell depletion. Clinical & Experimental Immunology, 138(3), 476-483.


Specifications

  • Host Species
  • Rat
  • Derivation
  • Rat
  • Specificity
  • Human
  • Clone
  • BTI-322 (also known as Lo-CD2a)
  • Applications
  • FuncS
  • Related Disease
  • First-time kidney rejection, and resistant rejection

Applications

  • Application Notes
  • This antibody has been tested for use in Functional Assay.

Target

  • Alternative Names
  • CD2; CD2 molecule; T11; SRBC; LFA-2; T-cell surface antigen CD2; LFA-3 receptor; rosette receptor; erythrocyte receptor; lymphocyte-function antigen-2; T-cell surface antigen T11/Leu-5; CD2 antigen (p50), sheep red blood cell receptor

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Intrabody

CAT Product Name Application Type
IAB-B074(A) Recombinant Anti-human CD2 Intrabody [(D-Arg)9] PCA, WB, FuncS scFv-(D-Arg)9
IAB-B074(G) Recombinant Anti-human CD2 Intrabody [+36 GFP] ELISA, IF, Neut, FuncS scFv-(+36GFP)
IAB-B074(T) Recombinant Anti-human CD2 Intrabody [Tat] ELISA, Neut, FuncS scFv-Tat

Humanized Antibody

CAT Product Name Application Type
TAB-104 Anti-Human CD2 Recombinant Antibody (Siplizumab) ELISA, IP, FC, FuncS, Neut, IF, ICC IgG1 - kappa

Rat Antibody

CAT Product Name Application Type
TAB-110CL Anti-Human CD2 Recombinant Antibody (BTI-322) FuncS Antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-295CQ Mouse Anti-CD2 Recombinant Antibody (clone CBL975) WB, FC, Neut, IHC Mouse IgG1, κ
NEUT-296CQ Mouse Anti-CD2 Recombinant Antibody (clone RPA-2.10) WB, Neut, FC, IHC, IHC-Fr Mouse IgG1, κ
NEUT-297CQ Mouse Anti-CD2 Recombinant Antibody (clone TS1/8) Neut, FC, CyTOF Mouse IgG1
NEUT-299CQ Rat Anti-CD2 Recombinant Antibody (clone 12-15) Neut, FC, IP Rat IgG1, κ
NEUT-300CQ Rat Anti-CD2 Recombinant Antibody (clone RM2) FC, FuncS, Neut Rat IgG2b, λ

Blocking Antibody

CAT Product Name Application Type
NEUT-298CQ Mouse Anti-CD2 Recombinant Antibody (clone TS218) Block, FC, IP, WB Mouse IgG1, κ
NEUT-301CQ Rat Anti-CD2 Recombinant Antibody (clone RM2-5) FC, IP, Block, Costim Rat IgG2b, λ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0542 Hi-Affi™ Rabbit Anti-CD2 Recombinant Antibody (clone DS542AB) WB, IHC, ICC, IP Rabbit IgG

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0019-LSX-S(P) Human Anti-CD2 Recombinant Antibody (clone 35.1); scFv Fragment ELISA Mouse scFv

Fab Fragment Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-104 Afuco™ Anti-CD2 ADCC Recombinant Antibody (Siplizumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody

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