Mouse Anti-NHP CD2 Recombinant Antibody (clone RPA-2.10) (CAT#: VS-1024-XY84)
Engineered from mouse sources, this monoclonal antibody is finely tuned to target CD2 with exceptional specificity. It exhibits reactivity across chimpanzee, human, non-human primate, and pig samples, thanks to its derivation from a recombinant full-length human CD2 protein. Optimized for immunofluorescence (IF) and flow cytometry (FC), this antibody offers superior performance in detecting and analyzing CD2 expression, making it an indispensable tool for advanced immunological research and cell analysis.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line JURKAT
(Immunofluorescent staining of human cell line JURKAT shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/2036_G3_1_selected.jpg
(Immunofluorescent staining of human cell line JURKAT shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/2036_G3_1_selected.jpg
Figure 2 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/11413_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/11413_A_8_8.jpg
Figure 3 Tonsil
(Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/11413_A_4_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/11413_A_4_8.jpg
Figure 4 RNA cell line category: Group enriched (HDLM-2, JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116824-CD2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116824-CD2
Figure 4 RNA cell line category: Group enriched (HDLM-2, JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116824-CD2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116824-CD2
Specifications
- Immunogen
- Recombinant full-length human CD2 protein
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- CD2
- Species Reactivity
- Chimpanzee, Human, Non-human primate, Pig
- Clone
- RPA-2.10
- Applications
- IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.05% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- IF: 1-2 µg/mL, FC: 1-2 µg/106 cells
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B074(A) | Recombinant Anti-human CD2 Intrabody [(D-Arg)9] | PCA, WB, FuncS | scFv-(D-Arg)9 |
| IAB-B074(G) | Recombinant Anti-human CD2 Intrabody [+36 GFP] | ELISA, IF, Neut, FuncS | scFv-(+36GFP) |
| IAB-B074(T) | Recombinant Anti-human CD2 Intrabody [Tat] | ELISA, Neut, FuncS | scFv-Tat |
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