CytoStream™ Rabbit Anti-CD2 Recombinant Antibody (VS13-YC155)
CAT#: VS13-YC155
The CytoStream™ Rabbit Anti-CD2 Recombinant Antibody is specific for human CD2. CD2, involved in immune signaling, is engaged in transmembrane signal pathways, impacting diseases like squamous cell carcinoma by affecting receptor activity. The anti-CD2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/2036_G3_1_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/11413_A_8_8.jpg
Normal Tissue
Figure 3 Tonsil
Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/11413_A_4_8.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (HDLM-2, JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116824-CD2
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Specifications
- Immunogen
- Recombinant human CD2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD2
- Species Reactivity
- Human
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Conjugate
- NONE
- Related Disease
- Penis Squamous Cell Carcinoma and Immune Defect
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/50
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- CD2 Molecule; CD2 Antigen (P50), Sheep Red Blood Cell Receptor; T-Cell Surface Antigen T11/Leu-5; Erythrocyte Receptor; Rosette Receptor; LFA-3 Receptor; LFA-2;
- Gene ID
- 914
- UniProt ID
- P06729
- Long Name
- CD2 Molecule
- Cellular Localization
- Cell membrane; Membrane
- Protein Refseq
- NP_001315538.1; NP_001758.2
- Function
- CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| HPAB-0019-LSX-F(E) | Human Anti-CD2 Recombinant Antibody (clone 35.1); Fab Fragment | ELISA | Mouse Fab |
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| AFC-TAB-104 | Afuco™ Anti-CD2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-104) | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0225-XY21 | CytoStream™ Mouse Anti-CD2 Recombinant Antibody (clone RPA-2.10) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY80 | Mouse Anti-CD2 (clone 35.1) scFv-Fc Chimera | ELISA, Inhib | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC276 | Recombinant Anti-CD2 Vesicular Antibody, EV Displayed (VS-0425-YC276) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1134 | Anti-CD2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1135 | Anti-Mouse CD2 Immunohistochemistry Kit | IHC |
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