CytoStream™ Rabbit Anti-CD2 Recombinant Antibody (VS13-YC155) (CAT#: VS13-YC155)
The CytoStream™ Rabbit Anti-CD2 Recombinant Antibody is specific for human CD2. CD2, involved in immune signaling, is engaged in transmembrane signal pathways, impacting diseases like squamous cell carcinoma by affecting receptor activity. The anti-CD2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line JURKAT
(Immunofluorescent staining of human cell line JURKAT shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/2036_G3_1_selected.jpg
(Immunofluorescent staining of human cell line JURKAT shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/2036_G3_1_selected.jpg
Figure 2 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/11413_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/11413_A_8_8.jpg
Figure 3 Tonsil
(Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/11413_A_4_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3883/11413_A_4_8.jpg
Figure 4 RNA cell line category: Group enriched (HDLM-2, JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116824-CD2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116824-CD2
Figure 4 RNA cell line category: Group enriched (HDLM-2, JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116824-CD2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116824-CD2
Specifications
- Immunogen
- Recombinant human CD2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD2
- Species Reactivity
- Human
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Conjugate
- NONE
- Related Disease
- Penis Squamous Cell Carcinoma and Immune Defect
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/50
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- CD2 Molecule; CD2 Antigen (P50), Sheep Red Blood Cell Receptor; T-Cell Surface Antigen T11/Leu-5; Erythrocyte Receptor; Rosette Receptor; LFA-3 Receptor; LFA-2;
- Gene ID
- 914
- UniProt ID
- P06729
- Long Name
- CD2 Molecule
- Cellular Localization
- Cell membrane; Membrane
- Protein Refseq
- NP_001315538.1; NP_001758.2
- Function
- CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B074(A) | Recombinant Anti-human CD2 Intrabody [(D-Arg)9] | PCA, WB, FuncS | scFv-(D-Arg)9 |
| IAB-B074(G) | Recombinant Anti-human CD2 Intrabody [+36 GFP] | ELISA, IF, Neut, FuncS | scFv-(+36GFP) |
| IAB-B074(T) | Recombinant Anti-human CD2 Intrabody [Tat] | ELISA, Neut, FuncS | scFv-Tat |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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