Anti-Human CEACAM5 Recombinant Antibody (A5B7)
CAT#: TAB-408LC
Recombinant monoclonal antibody to CEACAM5. A5B7 is a mouse monoclonal antibody that can be potentially used in the treatment of cancer.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19758/1660_G5_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11041/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Gradient
Gradient: Ascending
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_7_3.jpg
Normal Tissue
Figure 4 Rectum
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_6_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
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SDS-PAGE
Figure 1 Anti-Human CEACAM5 Recombinant Antibody (TAB-408LC) in SDS-PAGE
SDS-PAGE analysis of TAB-408LC in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions.
SEC-HPLC
Figure 2 Anti-Human CEACAM5 Recombinant Antibody (TAB-408LC) in SEC-HPLC
The purity of TAB-408LC was 96.91% as determined by SEC-HPLC.
WB
Figure 3 Anti-Human CEACAM5 Recombinant Antibody (TAB-408LC) in WB
Western blot analysis of TAB-408LC was performed by loading human CEACAM5 protein, His tag.
ELISA
Figure 4 Anti-Human CEACAM5 Recombinant Antibody (TAB-408LC) in ELISA
ELISA analysis of TAB-408LC was performed by coating with human CEACAM5 protein (His tag). Then blocked with BSA and incubated with TAB-408LC. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Host Species
- Mouse
- Specificity
- Human
- Clone
- A5B7
- Related Disease
- Cancer
Product Property
- Preservative
- Human
- Storage
- CEA (carcinoembryonic antigen)
Target
- Alternative Names
- CEACAM5; carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; meconium antigen 100;
- Gene ID
- 1048
- UniProt ID
- A0A024R0K5
REVIEWS AND Q&AS
CITATIONS
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Highly reliable reagent
We used A5B7 in several immunohistochemistry experiments, and it provided consistent staining patterns. The detailed staining allowed us to draw meaningful conclusions regarding CEACAM5 expression.
User-friendly datasheet
The accompanying datasheet was very comprehensive and provided all necessary instructions for use, storage, and dilution. It eased the setup of our experiments significantly.
Efficient CEACAM5 detection
In our immunofluorescence studies, A5B7 provided bright and distinct localization of CEACAM5, helping us map its distribution accurately within the samples.
M**k01.Mar,25
Excellent for WB
We have utilized the Anti - Human CEACAM5 Recombinant Antibody (A5B7) in Western blotting (WB) experiments on protein lysates from human colon polyps, metastatic colorectal cancer tissues, and normal colon epithelial cells. The antibody showed outstanding sensitivity. It clearly detected CEACAM5, even at low expression levels, and revealed distinct band patterns. This was extremely useful for studying CEACAM5 - related signaling pathways in colorectal cancer.
Q&As
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What is the primary target of Anti-Human CEACAM5 Recombinant Antibody (A5B7)?
A: The primary target of A5B7 is CD66e, also known as CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5), which is often overexpressed in certain cancers.
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How does A5B7 bind to its target?
A: The A5B7 antibody recognizes and binds to a specific epitope on the CEACAM5 protein, facilitating downstream processes such as signal transduction or marking the cell for further action.
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Can A5B7 be used in combination with other antibodies?
A: Yes, A5B7 can be used in multiplex assays or in combination with other antibodies, provided they do not compete for the same binding site or interfere with each other.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-408LC, RRID: AB_3111927)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone A5B7"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L003E | anti-CEACAM5 immunotoxin BDI-1 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L003R | anti-CEACAM5 immunotoxin BDI-1 (IgG)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L004E | anti-CEACAM5 immunotoxin BDI-1 (scFv)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L004R | anti-CEACAM5 immunotoxin BDI-1 (scFv)-RTA | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-409LC | Human Anti-CEACAM5 Recombinant Antibody (TAB-409LC) | SPR, ELISA, FC | Humanized antibody |
| TAB-412LC | Human Anti-CEACAM5 Recombinant Antibody (TAB-412LC) | ELISA, FC | Chimeric (Mouse/Human) IgG1 |
| TAB-409LC-S(P) | Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-409LC-S(P)) | SPR, ELISA, FC | Humanized scFv |
| TAB-412LC-S(P) | Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-412LC-S(P)) | FC, ELISA | Human scFv |
| TAB-413LC-S(P) | Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-413LC-S(P)) | FC, ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-411LC-S(P) | Human Anti-Human CEACAM5 Recombinant Antibody scFv Fragment | ELISA | Human antibody |
| TAB-411LC-F(E) | Human Anti-Human CEACAM5 Recombinant Antibody Fab Fragment | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-410LC-F(E) | Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-410LC-F(E)) | ELISA | Humanized Fab |
| TAB-414LC-F(E) | Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-414LC-F(E)) | FC, ELISA | Humanized Fab |
| TAB-415LC-F(E) | Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-415LC-F(E)) | FC, ELISA | Humanized Fab |
| TAB-416LC-F(E) | Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-416LC-F(E)) | FC, ELISA | Humanized Fab |
| TAB-417LC-F(E) | Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-417LC-F(E)) | IF, ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-002LC-VHH | Anti-Human CEACAM5 Recombinant Antibody(VHH library) | SPR, FC, ELISA | Single domain antibody |
| HPAB-0204-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0204-FY) | ADCC, ELISA | llama VHH |
| HPAB-0205-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0205-FY) | ADCC, E | llama VHH |
| HPAB-0206-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0206-FY) | ADCC, E | llama VHH |
| HPAB-1024WJ | Recombinant Camelid Anti-CEACAM5 Single Domain Antibody | SPR, Inhib | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0089MZ | Recombinant Mouse Anti-Carcinoembryonic antigen Antibody (clone A9203) | ELISA | Mouse antibody |
| VS7-HM694 | Mouse Anti-CEACAM5 Recombinant Antibody (clone 3B9C9) | FCM | Mouse IgG2a |
| VS7-HM695 | Mouse Anti-CEACAM5 Recombinant Antibody (clone 6G12F11 ) | IHC | Mouse IgG1 |
| VS7-HM696 | Mouse Anti-CEACAM5 Recombinant Antibody (clone CBL151HM) | WB, IF, ICC, ELISA | Mouse IgG |
| VS7-HM697 | Mouse Anti-CEACAM5 Recombinant Antibody (clone CBL152HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0118MZ | Chicken Anti-CEACAM5 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC771 | A*0201/Human CEA (YLSGANLNL) MHC Tetramer | FCM | |
| MHC-LC772 | A*2402/Human CEA (TYACFVSNL) MHC Tetramer | FCM | |
| MHC-LC2749 | PE-A*02:01/Human CEACAM5 (IMIGVLVGV) MHC Tetramer | FCM | |
| MHC-YF053 | DRB1*04:01/Human CEA (YACFVSNLATGRNNS) MHC Monomer | MHC Multimer | |
| MHC-YF059 | DRB1*04:05/Human CEA (LWWVNNQSLPVSP) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0649 | Hi-Affi™ Rabbit Anti-CEACAM5 Recombinant Antibody (clone DS649AB) | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0155-FY-S(P) | Mouse Anti-CEACAM5 Recombinant Antibody; scFv Fragment (HPAB-0155-FY-S(P)) | Activ | Humanized scFv |
| HPAB-0243-FY-S(P) | Mouse Anti-CEACAM5 Recombinant Antibody scFv Fragment (HPAB-0243-FY-S(P)) | ELISA | Mouse scFv |
| HPAB-0348-YJ-S(P) | Mouse Anti-CEACAM5 Recombinant Antibody (clone 10E1); scFv Fragment | ELISA, IHC | Mouse scFv |
| HPAB-1094WJ-S(P) | Mouse Anti-CEACAM5 Recombinant Antibody; scFv Fragment (HPAB-1094WJ-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-2106-FY-S(P) | Human Anti-CEACAM5 Recombinant Antibody (clone CEA6); scFv Fragment | ELISA, FC, Inhib | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0155-FY-F(E) | Mouse Anti-CEACAM5 Recombinant Antibody; Fab Fragment (HPAB-0155-FY-F(E)) | Activ | Humanized Fab |
| HPAB-0243-FY-F(E) | Human Anti-CEACAM5 Recombinant Antibody Fab Fragment (HPAB-0243-FY-F(E)) | ELISA | Chimeric (mouse/human) Fab |
| HPAB-0348-YJ-F(E) | Mouse Anti-CEACAM5 Recombinant Antibody (clone 10E1); Fab Fragment | ELISA, IHC | Mouse Fab |
| HPAB-1094WJ-F(E) | Mouse Anti-CEACAM5 Recombinant Antibody; Fab Fragment (HPAB-1094WJ-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-2106-FY-F(E) | Human Anti-CEACAM5 Recombinant Antibody (clone CEA6); Fab Fragment | ELISA, FC, Inhib | Human Fab, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-703 | Afuco™ Anti-CEACAM5 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-703) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY62 | Mouse Anti-NHP CEACAM5 Recombinant Antibody (clone SPM506) | IHC, IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY29 | Human Anti-CEACAM5 (clone MFE-23) scFv-Fc Chimera | BI | Human IgG1, scFv-Fc |
| VS-0125-FY89 | Human Anti-CEACAM5 (clone A5B7) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC97 | Recombinant Anti-CEACAM5 Vesicular Antibody, EV Displayed (VS-0425-YC97) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1355 | Anti-CEACAM5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1356 | Anti-Human CEACAM5 Immunohistochemistry Kit | IHC |
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