Mouse Anti-F8 Recombinant Antibody (clone 3E6) (CAT#: PABL-051)
Recombinant Mouse Antibody (3E6) is capable of binding to Coagulation factor VIII, expressed in Chinese Hamster Ovary cells (CHO).
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ELISA
Figure 1 ELISA of C2-mAb interactions.
Binding of His6-C2 to immobilized 3E6 IgG (●) and G99 IgG (○). Bound His6-C2 was detected using the Ni-NTA-alkaline phosphatase/pNPP system.
Walter, J. D., Werther, R. A., Polozova, M. S., Pohlman, J., Healey, J. F., Meeks, S. L., ... & Spiegel, P. C. (2013). Characterization and solution structure of the factor VIII C2 domain in a ternary complex with classical and non-classical inhibitor antibodies. Journal of Biological Chemistry, 288(14), 9905-9914.
Inhib
Figure 2 Bethesda assays for inhibition of fVIII activity in plasma.
Pooled normal human plasma was successively diluted with IgG or Fab, and one-stage coagulation activity was measured. Inhibition of fVIII activity by 3E6 whole IgG (○) and 3E6 Fab (●).
Walter, J. D., Werther, R. A., Polozova, M. S., Pohlman, J., Healey, J. F., Meeks, S. L., ... & Spiegel, P. C. (2013). Characterization and solution structure of the factor VIII C2 domain in a ternary complex with classical and non-classical inhibitor antibodies. Journal of Biological Chemistry, 288(14), 9905-9914.
Specifications
- Immunogen
- Human coagulation factor VIII, procoagulant component
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human F8
- Species Reactivity
- Human
- Clone
- 3E6
- Applications
- ELISA, WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay and Inhibition.
Target
- Alternative Names
- F8; coagulation factor VIII; procoagulant component; AHF; F8B; F8C; HEMA; FVIII; DXS1253E; coagulation factor VIII; factor VIII F8B; antihemophilic factor; coagulation factor VIIIc
- Gene ID
- 2157
- UniProt ID
- P00451
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-930-VHH | Recombinant Anti-Human F8 VHH Single Domain Antibody | ELISA, Neut, ICC, FC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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