What are the source and isotype details of this Factor VIII antibody?

This is a Recombinant Human Anti-F8 Antibody Fab Fragment, clone BO2C11. It is derived from a patient memory B-lymphocyte cell line and is an IgG4 kappa isotype. It is designed to specifically bind to the C2 domain of Factor VIII, which is involved in the blood coagulation cascade.

Can this antibody be used for functional inhibition studies?

Yes, this antibody is suitable for Functional Studies (FuncS). The clone BO2C11 is known to inhibit the binding of Factor VIII to von Willebrand factor and phospholipids. This characteristic makes it a valuable tool for researching the intrinsic pathway of blood coagulation and inhibitor mechanisms.

Recombinant Human Anti-F8 Antibody Fab Fragment (BO2C11)

CAT#: PABX-058-F (E)

Anti-F8 antibody is a Human of Fab class that binds to an F8. It developed From patient memory B-lymphocyte cell line.

Gene Expression

Normal Tissue Figure 1 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_7_3.jpg

Normal Tissue Figure 2 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_7_5.jpg

Normal Tissue Figure 3 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_4_6.jpg

RNA Expression Figure 4 RNA cell line category:Cell line enhanced (U-2197)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185010-F8

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
Fab

Antibody Isotype
IgG4 κ

Specificity
Tested positive against native Human F8

Species Reactivity
Human

Clone
BO2C11

Applications
Can be useful in applications such as: Western blot; Enzyme-linked Immunosorbent Assay; Functional Study

Product Property

Purity
>95% by SDS-PAGE and HPLC analysis

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

Alternative Names
F8; coagulation factor VIII; procoagulant component; AHF; F8B; F8C; HEMA; FVIII; DXS1253E; coagulation factor VIII; factor VIII F8B; antihemophilic factor; coagulation factor VIIIc; TB-402; Krix-1

Gene ID
2157

UniProt ID
P00451

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Accurate F8 Detection in WB

We employed this Fab fragment, clone BO2C11, for Western Blotting to analyze Factor VIII expression levels in cell lysates. The antibody recognized the F8 protein specifically, and because it is a Fab fragment, we observed reduced background noise compared to some full-length antibodies we have tried previously.

Effective for Functional Studies

Our lab focuses on the intrinsic pathway of blood coagulation, and we used this antibody in functional studies involving Factor VIII. The product displayed high affinity and specificity, allowing us to effectively model the interactions of F8 with Factor IXa in our controlled in vitro experiments.

Q&As

What are the source and isotype details of this Factor VIII antibody?

A: This is a Recombinant Human Anti-F8 Antibody Fab Fragment, clone BO2C11. It is derived from a patient memory B-lymphocyte cell line and is an IgG4 kappa isotype. It is designed to specifically bind to the C2 domain of Factor VIII, which is involved in the blood coagulation cascade.
Can this antibody be used for functional inhibition studies?

A: Yes, this antibody is suitable for Functional Studies (FuncS). The clone BO2C11 is known to inhibit the binding of Factor VIII to von Willebrand factor and phospholipids. This characteristic makes it a valuable tool for researching the intrinsic pathway of blood coagulation and inhibitor mechanisms.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABX-058-F (E).pdf

MSDS

MSDS - PABX-058-F (E).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

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CAT
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