Mouse Anti-FAP Recombinant Antibody (HPAB-0171-CN) (CAT#: HPAB-0171-CN)
This product is a recombinant mouse antibody that recognizes FAP. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Specific Inquiry
SDS-PAGE
Figure 1 Mouse Anti-FAP Recombinant Antibody (HPAB-0171-CN) in SDS-PAGE
Lane 1: Non-reduced mAb
Lane 2: Reduced mAb
WB
Figure 2 Mouse Anti-FAP Recombinant Antibody (HPAB-0171-CN) in WB
Western blot analysis of HPAB-0171-CN was performed with Recombinant Human FAP Protein (His Tag).
HPAB-0171-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing antigen (0.1μg)
Lane 2: Reducing antigen (0.3μg)
Lane 3: Reducing antigen (0.6μg)
ELISA
Figure 3 Mouse Anti-FAP Recombinant Antibody (HPAB-0171-CN) in ELISA
ELISA analysis of HPAB-0171-CN was performed with Recombinant Human FAP Protein (His Tag).
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
WB
Figure 4 Mouse Anti-FAP Recombinant Antibody (HPAB-0171-CN) in WB
Western blot analysis of HPAB-0171-CN was performed with Recombinant Mouse FAP Protein (His Tag).
HPAB-0171-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing antigen (0.1μg)
Lane 2: Reducing antigen (0.3μg)
Lane 3: Reducing antigen (0.6μg)
ELISA
Figure 5 Mouse Anti-FAP Recombinant Antibody (HPAB-0171-CN) in ELISA
ELISA analysis of HPAB-0171-CN was performed with Recombinant Mouse FAP Protein (His Tag).
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Specifications
- Immunogen
- CD8-murine FAP fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human FAP
- Species Reactivity
- Human, Mouse
- Applications
- ELISA
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2277z | Mouse Anti-FAP Recombinant Antibody (clone 36B12) | FC, IF, IP | Mouse IgG1 |
| HPAB-0170-CN | Human Anti-FAP Recombinant Antibody (HPAB-0170-CN) | ELISA, IF | Human IgG |
| HPAB-0173-CN | Human Anti-FAP Recombinant Antibody (HPAB-0173-CN) | ELISA | Humanized IgG1, κ |
| HPAB-2061-FY | Human Anti-FAP Recombinant Antibody (HPAB-2061-FY) | SPR, IHC | Human IgG1 |
| HPAB-2062-FY | Human Anti-FAP Recombinant Antibody (HPAB-2062-FY) | FC | Human IgG1 |
Customer Reviews and Q&As
Customer Review
Q&As
-
Reliable in Western Blot
In Western Blotting, this antibody consistently yields positive results. The data interpretation process is made simple by the distinct and unambiguous bands. -
Excellent Specificity
Excellent specificity for FAP is demonstrated by the antibody, with little background noise. ideal for my purposes in research. -
Easy to Use
The antibody worked as anticipated in ELISA tests, and the instructions were simple to follow.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0171-CN, RRID: AB_3111364)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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